{"created":"2021-03-01T06:22:11.391984+00:00","id":5492,"links":{},"metadata":{"_buckets":{"deposit":"8ed589ae-066b-433d-af35-b308e4836735"},"_deposit":{"id":"5492","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5492"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00005492","sets":["75:275:400","9:233:280"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"細胞分化の準備期に機能するLatent process遺伝子群の同定と、神経突起伸長レベルのデコーダーとしての機能解析"}]},"item_7_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03-22","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_7_date_granted_25":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2012-03-22"}]},"item_7_degree_grantor_23":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"University of Tokyo (東京大学)"}]}]},"item_7_degree_name_20":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(理学)"}]},"item_7_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A latent process involving signal transduction and gene expression is needed as a preparation step for cellular function. We previously found that nerve growth factor (NGF)-induced cell differentiation has a latent process, which is dependent on ERK activity and gene expression and required for subsequent neurite extension. A latent process can be considered a preparation step that decodes extracellular stimulus information into cellular functions; however, molecular mechanisms of this process remain unknown. I identified Metrnl, Dclk1, and Serpinb1a as latent process (LP) genes that are induced during the latent process with distinct temporal expression profiles and are required for subsequent neurite extension in PC12 cells. The LP gene products showed distinct spatial localization. Overexpression of all LP genes together, but not of each gene separately, enhanced NGF-induced neurite extension. The LP genes showed distinct dependency on the duration of ERK activity, and they were also induced during the latent process of pituitary adenylate cyclase-activating polypeptide (PACAP)- and forskolin-induced cell differentiation. Regardless of neurotrophic factors, expression levels of the LP genes during the latent process (0-12 h), but not phosphorylation levels of ERK, always correlated with subsequent neurite extension length (12-24 h). Thus, the LP genes appeared to be the common decoders for neurite extension length regardless of neurotrophic factors, and they may function in distinct temporal and spatial manners during the latent process. Our findings provide molecular insight into the physiological meaning of the latent process as the preparation step for decoding information for future phenotypic change.","subitem_description_type":"Abstract"}]},"item_7_dissertation_number_26":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第27837号"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11442","nameIdentifierScheme":"WEKO"}],"names":[{"name":"渡邉, 可奈子"}]}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15083/00005483","subitem_identifier_reg_type":"JaLC"}]},"item_7_select_21":{"attribute_name":"学位","attribute_value_mlt":[{"subitem_select_item":"doctoral"}]},"item_7_subject_13":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"463","subitem_subject_scheme":"NDC"}]},"item_7_text_22":{"attribute_name":"学位分野","attribute_value_mlt":[{"subitem_text_value":"Science(Rigaku)(理学)"}]},"item_7_text_24":{"attribute_name":"研究科・専攻","attribute_value_mlt":[{"subitem_text_value":"Department of Biophysics and Biochemistry, Graduate School of Science (理学系研究科生物化学専攻)"}]},"item_7_text_27":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_value":"博理第5840号"}]},"item_7_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院理学系研究科生物化学専攻"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Watanabe, Kanako"}],"nameIdentifiers":[{"nameIdentifier":"11441","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-01"}],"displaytype":"detail","filename":"Thesis_watanabekanako.pdf","filesize":[{"value":"5.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Thesis_watanabekanako.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/5492/files/Thesis_watanabekanako.pdf"},"version_id":"1781c0d3-b621-4c31-8f2f-90e5ccab011c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Latent process genes for cell differentiation are common decoders of neurite extension length","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Latent process genes for cell differentiation are common decoders of neurite extension length"}]},"item_type_id":"7","owner":"1","path":["280","400"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-02-24"},"publish_date":"2014-02-24","publish_status":"0","recid":"5492","relation_version_is_last":true,"title":["Latent process genes for cell differentiation are common decoders of neurite extension length"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T03:47:04.872442+00:00"}