{"created":"2021-03-01T06:22:17.677140+00:00","id":5594,"links":{},"metadata":{"_buckets":{"deposit":"0bb4f8a4-8265-4a2b-9687-4be50b906030"},"_deposit":{"id":"5594","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5594"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00005594"},"item_7_biblio_info_7":{"attribute_name":"\u66f8\u8a8c\u60c5\u5831","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-06-27","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_7_date_granted_25":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5","attribute_value_mlt":[{"subitem_dategranted":"2011-06-27"}]},"item_7_degree_grantor_23":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u6a5f\u95a2","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"University of Tokyo (\u6771\u4eac\u5927\u5b66)"}]}]},"item_7_degree_name_20":{"attribute_name":"\u5b66\u4f4d\u540d","attribute_value_mlt":[{"subitem_degreename":"\u535a\u58eb(\u751f\u547d\u79d1\u5b66)"}]},"item_7_description_5":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"Mrc1/Claspin are evolutionally conserved and has been known to play a major role in replication checkpoint regulation. Furthermore, checkpoint-independent roles at a replication fork have been suggested in yeasts. However, the information on biochemical functions of Claspin has been limited, due to difficulties associated with purification of this protein. I have improved expression and purification procedures for human Claspin as well as other high molecular weight replication proteins. I used a CSII-EF-MCS expression vector and Polyethyleneimine (PEI) as a transfection reagent for high-level expression in 293T cells. This method could be generally applicable to purification of large-sized proteins which have been difficult to handle. Purified Claspin stimulated the helicase activity of the MCM4-6-7 complex in vitro. This finding can at least partially explain a stimulatory role of Claspin at a replication fork. I also identified interactions of Claspin with replication and checkpoint factors, which may bear physiological significance.","subitem_description_type":"Abstract"}]},"item_7_dissertation_number_26":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7","attribute_value_mlt":[{"subitem_dissertationnumber":"\u7532\u7b2c27368\u53f7"}]},"item_7_full_name_3":{"attribute_name":"\u8457\u8005\u5225\u540d","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11646","nameIdentifierScheme":"WEKO"}],"names":[{"name":"\u5b87\u91ce, \u4fee\u53f8"}]}]},"item_7_identifier_registration":{"attribute_name":"ID\u767b\u9332","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15083/00005585","subitem_identifier_reg_type":"JaLC"}]},"item_7_select_21":{"attribute_name":"\u5b66\u4f4d","attribute_value_mlt":[{"subitem_select_item":"doctoral"}]},"item_7_subject_13":{"attribute_name":"\u65e5\u672c\u5341\u9032\u5206\u985e\u6cd5","attribute_value_mlt":[{"subitem_subject":"467","subitem_subject_scheme":"NDC"}]},"item_7_text_22":{"attribute_name":"\u5b66\u4f4d\u5206\u91ce","attribute_value_mlt":[{"subitem_text_value":"Integrated Biosciences (\u751f\u547d\u79d1\u5b66)"}]},"item_7_text_24":{"attribute_name":"\u7814\u7a76\u79d1\u30fb\u5c02\u653b","attribute_value_mlt":[{"subitem_text_value":"Department of Medical Genome Sciences, Graduate School of Frontier Sciences (\u65b0\u9818\u57df\u5275\u6210\u79d1\u5b66\u7814\u7a76\u79d1\u30e1\u30c7\u30a3\u30ab\u30eb\u30b2\u30ce\u30e0\u5c02\u653b)"}]},"item_7_text_27":{"attribute_name":"\u5b66\u4f4d\u8a18\u756a\u53f7","attribute_value_mlt":[{"subitem_text_value":"\u535a\u5275\u57df\u7b2c711\u53f7"}]},"item_7_text_4":{"attribute_name":"\u8457\u8005\u6240\u5c5e","attribute_value_mlt":[{"subitem_text_value":"\u6771\u4eac\u5927\u5b66\u5927\u5b66\u9662\u65b0\u9818\u57df\u5275\u6210\u79d1\u5b66\u7814\u7a76\u79d1\u751f\u547d\u79d1\u5b66\u7814\u7a76\u7cfb\u30e1\u30c7\u30a3\u30ab\u30eb\u30b2\u30ce\u30e0\u5c02\u653b"},{"subitem_text_value":"Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Uno, Syuzi"}],"nameIdentifiers":[{"nameIdentifier":"11645","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-01"}],"displaytype":"detail","filename":"K-03492.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"K-03492.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/5594/files/K-03492.pdf"},"version_id":"04ab7dbb-7c24-4041-aff9-8969698d080c"}]},"item_keyword":{"attribute_name":"\u30ad\u30fc\u30ef\u30fc\u30c9","attribute_value_mlt":[{"subitem_subject":"DNA replication","subitem_subject_scheme":"Other"},{"subitem_subject":"checkpoint","subitem_subject_scheme":"Other"},{"subitem_subject":"Claspin","subitem_subject_scheme":"Other"},{"subitem_subject":"expression","subitem_subject_scheme":"Other"},{"subitem_subject":"purification","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"\u52d5\u7269\u7d30\u80de\u3092\u7528\u3044\u305fDNA\u8907\u88fd/\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u56e0\u5b50Claspin\u306e\u767a\u73fe\u3001\u7cbe\u88fd\u3068\u6a5f\u80fd\u89e3\u6790","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"\u52d5\u7269\u7d30\u80de\u3092\u7528\u3044\u305fDNA\u8907\u88fd/\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u56e0\u5b50Claspin\u306e\u767a\u73fe\u3001\u7cbe\u88fd\u3068\u6a5f\u80fd\u89e3\u6790"}]},"item_type_id":"7","owner":"1","path":["9/233/280","110/267/345"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2014-02-24"},"publish_date":"2014-02-24","publish_status":"0","recid":"5594","relation_version_is_last":true,"title":["\u52d5\u7269\u7d30\u80de\u3092\u7528\u3044\u305fDNA\u8907\u88fd/\u30c1\u30a7\u30c3\u30af\u30dd\u30a4\u30f3\u30c8\u56e0\u5b50Claspin\u306e\u767a\u73fe\u3001\u7cbe\u88fd\u3068\u6a5f\u80fd\u89e3\u6790"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-01T19:41:14.535401+00:00"}