{"created":"2021-03-01T06:22:19.642650+00:00","id":5625,"links":{},"metadata":{"_buckets":{"deposit":"9822cd43-fcd5-4ae2-ad9b-05800544314b"},"_deposit":{"id":"5625","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5625"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00005625","sets":["75:151:408","9:233:280"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"アフリカツメガエル初期眼発生における新規核膜タンパク質Nemp1 の解析"}]},"item_7_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-23","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_7_date_granted_25":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2009-03-23"}]},"item_7_degree_grantor_23":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"University of Tokyo (東京大学)"}]}]},"item_7_degree_name_20":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(理学)"}]},"item_7_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To clarify the molecular mechanisms of early brain development in vertebrates, I analyzed new candidate developmental regulator genes selected using systematic expression pattern screening with a Xenopus anterior neuroectoderm cDNA library. In this thesis, I describe the identification and characterization of a newly identified nuclear envelope localized protein, termed Nemp1 (nuclear envelope integral membrane protein 1). Nemp1 has a putative signal peptide and five transmembrane domains, but does not have any other known domains or motifs. nemp1 is expressed in the anterior neuroectoderm at the neurula stage and in the head region, predominantly in the eyes, at later stages. Using the digitonin permeabilization-immunostaining method that I developed, Nemp1 is suggested to be localized to the inner nuclear membrane (INM) with its evolutionarily conserved C-terminal region facing the nucleoplasm. In functional analyses for Nemp1, both overexpression by mRNA injection and knockdown by antisense morpholino oligo injection in Xenopus embryos reduced the expression of early eye marker genes, rax, tbx3, and pax6, at the neurula stage, and later resulted mainly in severe eye defects at the tailbud stage. By contrast, the expression of a forebrain/midbrain marker, otx2, and a pan-neural marker, sox2, was largely unaffected in these injected embryos. In addition, analysis of Nemp1 deletion constructs showed that signal peptide and transmembrane domains are absolutely necessary for both eye-reducing activity and nuclear envelope localization, and importantly nuclear envelope-localization of the C-terminal region is necessary for Nemp1 activity. These data leads to the possibility that Nemp1 functions at the inner nuclear membrane as a complex, which requires a proper stoichiometry. I sought Nemp1-interacting proteins and show that nemp1 is coexpressed with baf (barrier-toautointegration factor) in the eye anlagen, and that Nemp1 interacts with BAF through the BAF-binding site in the C-terminal region and this site is required for Nemp1 activity. These data suggest that Nemp1 is involved in the expression of eye marker genes by functioning at the INM at least partly through BAF. This is the first evidence for a role of a nuclear envelope protein in organ-specific gene expression during embryonic development.","subitem_description_type":"Abstract"}]},"item_7_dissertation_number_26":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第24506号"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11704","nameIdentifierScheme":"WEKO"}],"names":[{"name":"儘田, 博志"}]}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15083/00005616","subitem_identifier_reg_type":"JaLC"}]},"item_7_select_21":{"attribute_name":"学位","attribute_value_mlt":[{"subitem_select_item":"doctoral"}]},"item_7_text_22":{"attribute_name":"学位分野","attribute_value_mlt":[{"subitem_text_value":"Science (Rigaku)(理学)"}]},"item_7_text_24":{"attribute_name":"研究科・専攻","attribute_value_mlt":[{"subitem_text_value":"Department of Biological Sciences, Graduate School of Science (理学系研究科生物科学専攻)"}]},"item_7_text_27":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_value":"博理第5404号"}]},"item_7_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院理学系研究科 生物科学専攻"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mamada, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"11703","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-01"}],"displaytype":"detail","filename":"1236319641_15322.pdf","filesize":[{"value":"16.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1236319641_15322.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/5625/files/1236319641_15322.pdf"},"version_id":"841cf7ef-7356-4acf-849a-67a1bcc58945"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Analysis of a novel nuclear envelope protein, Nemp1, in early Xenopus eye development","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Analysis of a novel nuclear envelope protein, Nemp1, in early Xenopus eye development"}]},"item_type_id":"7","owner":"1","path":["280","408"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-02-24"},"publish_date":"2014-02-24","publish_status":"0","recid":"5625","relation_version_is_last":true,"title":["Analysis of a novel nuclear envelope protein, Nemp1, in early Xenopus eye development"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T03:47:12.339117+00:00"}