{"created":"2021-03-01T06:22:19.642650+00:00","id":5625,"links":{},"metadata":{"_buckets":{"deposit":"9822cd43-fcd5-4ae2-ad9b-05800544314b"},"_deposit":{"id":"5625","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5625"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00005625"},"item_7_alternative_title_1":{"attribute_name":"\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_alternative_title":"\u30a2\u30d5\u30ea\u30ab\u30c4\u30e1\u30ac\u30a8\u30eb\u521d\u671f\u773c\u767a\u751f\u306b\u304a\u3051\u308b\u65b0\u898f\u6838\u819c\u30bf\u30f3\u30d1\u30af\u8ceaNemp1 \u306e\u89e3\u6790"}]},"item_7_biblio_info_7":{"attribute_name":"\u66f8\u8a8c\u60c5\u5831","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-23","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_7_date_granted_25":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5","attribute_value_mlt":[{"subitem_dategranted":"2009-03-23"}]},"item_7_degree_grantor_23":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u6a5f\u95a2","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"University of Tokyo (\u6771\u4eac\u5927\u5b66)"}]}]},"item_7_degree_name_20":{"attribute_name":"\u5b66\u4f4d\u540d","attribute_value_mlt":[{"subitem_degreename":"\u535a\u58eb(\u7406\u5b66)"}]},"item_7_description_5":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"To clarify the molecular mechanisms of early brain development in vertebrates, I analyzed new candidate developmental regulator genes selected using systematic expression pattern screening with a Xenopus anterior neuroectoderm cDNA library. In this thesis, I describe the identification and characterization of a newly identified nuclear envelope localized protein, termed Nemp1 (nuclear envelope integral membrane protein 1). Nemp1 has a putative signal peptide and five transmembrane domains, but does not have any other known domains or motifs. nemp1 is expressed in the anterior neuroectoderm at the neurula stage and in the head region, predominantly in the eyes, at later stages. Using the digitonin permeabilization-immunostaining method that I developed, Nemp1 is suggested to be localized to the inner nuclear membrane (INM) with its evolutionarily conserved C-terminal region facing the nucleoplasm. In functional analyses for Nemp1, both overexpression by mRNA injection and knockdown by antisense morpholino oligo injection in Xenopus embryos reduced the expression of early eye marker genes, rax, tbx3, and pax6, at the neurula stage, and later resulted mainly in severe eye defects at the tailbud stage. By contrast, the expression of a forebrain/midbrain marker, otx2, and a pan-neural marker, sox2, was largely unaffected in these injected embryos. In addition, analysis of Nemp1 deletion constructs showed that signal peptide and transmembrane domains are absolutely necessary for both eye-reducing activity and nuclear envelope localization, and importantly nuclear envelope-localization of the C-terminal region is necessary for Nemp1 activity. These data leads to the possibility that Nemp1 functions at the inner nuclear membrane as a complex, which requires a proper stoichiometry. I sought Nemp1-interacting proteins and show that nemp1 is coexpressed with baf (barrier-toautointegration factor) in the eye anlagen, and that Nemp1 interacts with BAF through the BAF-binding site in the C-terminal region and this site is required for Nemp1 activity. These data suggest that Nemp1 is involved in the expression of eye marker genes by functioning at the INM at least partly through BAF. This is the first evidence for a role of a nuclear envelope protein in organ-specific gene expression during embryonic development.","subitem_description_type":"Abstract"}]},"item_7_dissertation_number_26":{"attribute_name":"\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7","attribute_value_mlt":[{"subitem_dissertationnumber":"\u7532\u7b2c24506\u53f7"}]},"item_7_full_name_3":{"attribute_name":"\u8457\u8005\u5225\u540d","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"11704","nameIdentifierScheme":"WEKO"}],"names":[{"name":"\u5118\u7530, \u535a\u5fd7"}]}]},"item_7_identifier_registration":{"attribute_name":"ID\u767b\u9332","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15083/00005616","subitem_identifier_reg_type":"JaLC"}]},"item_7_select_21":{"attribute_name":"\u5b66\u4f4d","attribute_value_mlt":[{"subitem_select_item":"doctoral"}]},"item_7_text_22":{"attribute_name":"\u5b66\u4f4d\u5206\u91ce","attribute_value_mlt":[{"subitem_text_value":"Science (Rigaku)(\u7406\u5b66)"}]},"item_7_text_24":{"attribute_name":"\u7814\u7a76\u79d1\u30fb\u5c02\u653b","attribute_value_mlt":[{"subitem_text_value":"Department of Biological Sciences, Graduate School of Science (\u7406\u5b66\u7cfb\u7814\u7a76\u79d1\u751f\u7269\u79d1\u5b66\u5c02\u653b)"}]},"item_7_text_27":{"attribute_name":"\u5b66\u4f4d\u8a18\u756a\u53f7","attribute_value_mlt":[{"subitem_text_value":"\u535a\u7406\u7b2c5404\u53f7"}]},"item_7_text_4":{"attribute_name":"\u8457\u8005\u6240\u5c5e","attribute_value_mlt":[{"subitem_text_value":"\u6771\u4eac\u5927\u5b66\u5927\u5b66\u9662\u7406\u5b66\u7cfb\u7814\u7a76\u79d1 \u751f\u7269\u79d1\u5b66\u5c02\u653b"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mamada, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"11703","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-01"}],"displaytype":"detail","filename":"1236319641_15322.pdf","filesize":[{"value":"16.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1236319641_15322.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/5625/files/1236319641_15322.pdf"},"version_id":"841cf7ef-7356-4acf-849a-67a1bcc58945"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Analysis of a novel nuclear envelope protein, Nemp1, in early Xenopus eye development","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"Analysis of a novel nuclear envelope protein, Nemp1, in early Xenopus eye development"}]},"item_type_id":"7","owner":"1","path":["9/233/280","75/151/408"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2014-02-24"},"publish_date":"2014-02-24","publish_status":"0","recid":"5625","relation_version_is_last":true,"title":["Analysis of a novel nuclear envelope protein, Nemp1, in early Xenopus eye development"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-01T19:41:54.802144+00:00"}