{"created":"2021-03-01T06:17:12.301546+00:00","id":661,"links":{},"metadata":{"_buckets":{"deposit":"eeb54050-24e8-4adc-b535-5bda808b0b18"},"_deposit":{"id":"661","owners":[],"pid":{"revision_id":0,"type":"depid","value":"661"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00000661","sets":["144:145","9:10:11"]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-06-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageStart":"e98737","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"WRKY transcription factors and mitogen-activated protein kinase (MAPK) cascades have been shown to play pivotal roles in the regulation of plant defense responses. We previously reported that OsWRKY53-overexpressing rice plants showed enhanced resistance to the rice blast fungus. In this study, we identified OsWRKY53 as a substrate of OsMPK3/OsMPK6, components of a fungal PAMP-responsive MAPK cascade in rice, and analyzed the effect of OsWRKY53 phosphorylation on the regulation of basal defense responses to a virulence race of rice blast fungus Magnaporthe oryzae strain Ina86-137. An in vitro phosphorylation assay revealed that the OsMPK3/OsMPK6 activated by OsMKK4 phosphorylated OsWRKY53 recombinant protein at its multiple clustered serine-proline residues (SP cluster). When OsWRKY53 was coexpressed with a constitutively active mutant of OsMKK4 in a transient reporter gene assay, the enhanced transactivation activity of OsWRKY53 was found to be dependent on phosphorylation of the SP cluster. Transgenic rice plants overexpressing a phospho-mimic mutant of OsWRKY53 (OsWRKY53SD) showed further-enhanced disease resistance to the blast fungus compared to native OsWRKY53-overexpressing rice plants, and a substantial number of defense-related genes, including pathogenesis-related protein genes, were more upregulated in the OsWRKY53SD-overexpressing plants compared to the OsWRKY53-overexpressing plants. These results strongly suggest that the OsMKK4-OsMPK3/OsMPK6 cascade regulates transactivation activity of OsWRKY53, and overexpression of the phospho-mimic mutant of OsWRKY53 results in a major change to the rice transcriptome at steady state that leads to activation of a defense response against the blast fungus in rice plants.","subitem_description_type":"Abstract"}]},"item_2_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"UTokyo Research掲載「イネの耐病性向上に成功」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/","subitem_description_type":"Other"},{"subitem_description":"UTokyo Research \"Enhanced disease resistance in rice plants\" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/","subitem_description_type":"Other"}]},"item_2_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"PLOS"}]},"item_2_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1371/journal.pone.0098737","subitem_relation_type_select":"DOI"}}]},"item_2_relation_25":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0098737","subitem_relation_type_select":"URI"}}]},"item_2_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) 2014 Chujo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_2_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"Public Library of Science"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Biotechnology Research Center, The University of Tokyo"},{"subitem_text_value":"Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences"},{"subitem_text_value":"Department of Biosciences, Teikyo University, Utsunomiya"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chujo, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"2192","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyamoto, Koji"}],"nameIdentifiers":[{"nameIdentifier":"2193","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogawa, Satoshi"}],"nameIdentifiers":[{"nameIdentifier":"2194","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masuda, Yuka"}],"nameIdentifiers":[{"nameIdentifier":"2195","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Takafumi"}],"nameIdentifiers":[{"nameIdentifier":"2196","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kishi-Kaboshi, Mitsuko"}],"nameIdentifiers":[{"nameIdentifier":"2197","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Akira"}],"nameIdentifiers":[{"nameIdentifier":"2198","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishizawa, Yoko"}],"nameIdentifiers":[{"nameIdentifier":"2199","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Minami, Eiichi"}],"nameIdentifiers":[{"nameIdentifier":"2200","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nojiri, Hideaki"}],"nameIdentifiers":[{"nameIdentifier":"2201","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamane, Hisakazu"}],"nameIdentifiers":[{"nameIdentifier":"2202","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okada, Kazunori"}],"nameIdentifiers":[{"nameIdentifier":"2203","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-30"}],"displaytype":"detail","filename":"journal.pone.0098737.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"journal.pone.0098737.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/661/files/journal.pone.0098737.pdf"},"version_id":"84552a52-28e4-4116-8cc0-697c1c239314"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice"}]},"item_type_id":"2","owner":"1","path":["145","11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-02-04"},"publish_date":"2015-02-04","publish_status":"0","recid":"661","relation_version_is_last":true,"title":["Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T03:41:41.800250+00:00"}