{"created":"2021-03-01T06:17:14.574340+00:00","id":698,"links":{},"metadata":{"_buckets":{"deposit":"5d15e132-40b3-41ab-a343-303017b931a8"},"_deposit":{"id":"698","owners":[],"pid":{"revision_id":0,"type":"depid","value":"698"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00000698"},"item_2_biblio_info_7":{"attribute_name":"\u66f8\u8a8c\u60c5\u5831","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-06-01","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"10753","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_2_description_5":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"(R)-stereospecific amine transaminases (R-ATAs) are important biocatalysts for the production of (R)-amine compounds in a strict stereospecific manner. An improved R-ATA, ATA-117-Rd11, was successfully engineered for the manufacture of sitagliptin, a widely used therapeutic agent for type-2 diabetes. The effects of the individual mutations, however, have not yet been demonstrated due to the lack of experimentally determined structural information. Here we describe three crystal structures of the first isolated R-ATA, its G136F mutant and engineered ATA-117-Rd11, which indicated that the mutation introduced into the 136th residue altered the conformation of a loop next to the active site, resulting in a substrate-binding site with drastically modified volume, shape, and surface properties, to accommodate the large pro-sitagliptin ketone. Our findings provide a detailed explanation of the previously reported molecular engineering of ATA-117-Rd11 and propose that the loop near the active site is a new target for the rational design to change the substrate specificity of ATAs.","subitem_description_type":"Abstract"}]},"item_2_description_6":{"attribute_name":"\u5185\u5bb9\u8a18\u8ff0","attribute_value_mlt":[{"subitem_description":"UTokyo Research\u63b2\u8f09\u300c\u3055\u307e\u3056\u307e\u306a\u85ac\u306e\u5408\u6210\u306b\u91cd\u8981\u306a\u9175\u7d20\u304c\u4f5c\u7528\u3059\u308b\u76f8\u624b\u3092\u8a8d\u8b58\u3059\u308b\u4ed5\u7d44\u307f\u300d URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/how-an-important-enzyme-used-in-drug-production-recognizes-its-substrate.html","subitem_description_type":"Other"},{"subitem_description":"UTokyo Research \"How an important enzyme used in drug production recognizes its substrate\" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/how-an-important-enzyme-used-in-drug-production-recognizes-its-substrate.html","subitem_description_type":"Other"}]},"item_2_publisher_20":{"attribute_name":"\u51fa\u7248\u8005","attribute_value_mlt":[{"subitem_publisher":"Nature Publishing Group"}]},"item_2_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/srep10753","subitem_relation_type_select":"DOI"}}]},"item_2_relation_25":{"attribute_name":"\u95a2\u4fc2URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/how-an-important-enzyme-used-in-drug-production-recognizes-its-substrate.html","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/how-an-important-enzyme-used-in-drug-production-recognizes-its-substrate.html","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1038/srep10753","subitem_relation_type_select":"DOI"}}]},"item_2_rights_12":{"attribute_name":"\u6a29\u5229","attribute_value_mlt":[{"subitem_rights":"Copyright \u00a9 2015, Rights Managed by Nature Publishing Group"}]},"item_2_select_14":{"attribute_name":"\u8457\u8005\u7248\u30d5\u30e9\u30b0","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_2_text_4":{"attribute_name":"\u8457\u8005\u6240\u5c5e","attribute_value_mlt":[{"subitem_text_value":"Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Department of Ocean Sciences, Tokyo University of Marine Science and Technology"},{"subitem_text_value":"Biotechnology Development Laboratories, Kaneka Corporation"},{"subitem_text_value":"Research & Development Group, QOL Division, Kaneka Corporation"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Guan, Li-Jun"}],"nameIdentifiers":[{"nameIdentifier":"2570","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohtsuka, Jun"}],"nameIdentifiers":[{"nameIdentifier":"2571","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okai, Masahiko"}],"nameIdentifiers":[{"nameIdentifier":"2572","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyakawa, Takuya"}],"nameIdentifiers":[{"nameIdentifier":"2573","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mase, Tomoko"}],"nameIdentifiers":[{"nameIdentifier":"2574","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhi, Yuehua"}],"nameIdentifiers":[{"nameIdentifier":"2575","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hou, Feng"}],"nameIdentifiers":[{"nameIdentifier":"2576","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Noriyuki"}],"nameIdentifiers":[{"nameIdentifier":"2577","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwasaki, Akira"}],"nameIdentifiers":[{"nameIdentifier":"2578","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasohara, Yoshihiko"}],"nameIdentifiers":[{"nameIdentifier":"2579","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanokura, Masaru"}],"nameIdentifiers":[{"nameIdentifier":"2580","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-30"}],"displaytype":"detail","filename":"srep10753.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"srep10753.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/698/files/srep10753.pdf"},"version_id":"2a690e43-7143-4268-92e1-d16fbe75ddf1"}]},"item_keyword":{"attribute_name":"\u30ad\u30fc\u30ef\u30fc\u30c9","attribute_value_mlt":[{"subitem_subject":"Protein design","subitem_subject_scheme":"Other"},{"subitem_subject":"Biocatalysis","subitem_subject_scheme":"Other"},{"subitem_subject":"Transferases","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A new target region for changing the substrate specificity of amine transaminases","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"A new target region for changing the substrate specificity of amine transaminases"}]},"item_type_id":"2","owner":"1","path":["9/10/11","27/38/39"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2015-07-30"},"publish_date":"2015-07-30","publish_status":"0","recid":"698","relation_version_is_last":true,"title":["A new target region for changing the substrate specificity of amine transaminases"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-02T08:46:32.020496+00:00"}