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A simple measurement of the pH of root apoplast by the fluorescence ratio method
http://hdl.handle.net/2261/8137
http://hdl.handle.net/2261/813717c80b97-732d-4a21-bf7b-a1f8fc186791
名前 / ファイル | ライセンス | アクション |
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PR2_3.pdf (579.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-02-05 | |||||
タイトル | ||||||
タイトル | A simple measurement of the pH of root apoplast by the fluorescence ratio method | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Aluminum | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | apoplastic pH | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | fluorescence ratio method | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | Oregon Green | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | rice root | |||||
主題Scheme | Other | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Nishiyama, Hiroki
× Nishiyama, Hiroki× Ohya, Tomoyuki× Tanoi, Keitaro× Nakanishi, Tomoko M. |
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著者別名 | ||||||
識別子 | 105957 | |||||
識別子Scheme | WEKO | |||||
姓名 | 西山, 宏樹 | |||||
著者別名 | ||||||
識別子 | 105958 | |||||
識別子Scheme | WEKO | |||||
姓名 | 大矢, 智幸 | |||||
著者別名 | ||||||
識別子 | 105959 | |||||
識別子Scheme | WEKO | |||||
姓名 | 田野井, 慶太朗 | |||||
著者別名 | ||||||
識別子 | 105960 | |||||
識別子Scheme | WEKO | |||||
姓名 | 中西, 友子 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We present a simple procedure for measuring the pH in the apoplast of the rice root, using the fluorescent ratio method and Oregon Green 488 (OG). Staining with 5 μM OG for 3 min followed by 5-min washing with calcium solution was an effective method for measuring the pH of the apoplast at the root surface. In order to prevent the diffusion of OG from the apoplast, the circulation of a low concentration of OG (0.02 μM) in a root vessel was found to be a useful modality. However, aluminum ion disturbed pH measure-ment. In the presence of 50 μM AlCl3, the pH value was inaccurate at the pH rage higher than pH 5.0. | |||||
書誌情報 |
Plant Root 巻 2, p. 3-6, 発行日 2008 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18816754 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3117/plantroot.2.3 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
日本十進分類法 | ||||||
主題 | 471.1 | |||||
主題Scheme | NDC | |||||
出版者 | ||||||
出版者 | Japanese Society for Root Research | |||||
出版者別名 | ||||||
根研究会 |