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  1. 116 農学生命科学研究科・農学部
  2. 04 応用生命化学専攻
  3. 1160410 学術雑誌論文
  1. 0 資料タイプ別
  2. 10 学術雑誌論文
  3. 014 自然科学

A simple measurement of the pH of root apoplast by the fluorescence ratio method

http://hdl.handle.net/2261/8137
17c80b97-732d-4a21-bf7b-a1f8fc186791
名前 / ファイル ライセンス アクション
PR2_3.pdf PR2_3.pdf (579.9 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-02-05
タイトル
タイトル A simple measurement of the pH of root apoplast by the fluorescence ratio method
言語
言語 eng
キーワード
主題 Aluminum
主題Scheme Other
キーワード
主題 apoplastic pH
主題Scheme Other
キーワード
主題 fluorescence ratio method
主題Scheme Other
キーワード
主題 Oregon Green
主題Scheme Other
キーワード
主題 rice root
主題Scheme Other
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Nishiyama, Hiroki

× Nishiyama, Hiroki

WEKO 105953

Nishiyama, Hiroki

Search repository
Ohya, Tomoyuki

× Ohya, Tomoyuki

WEKO 105954

Ohya, Tomoyuki

Search repository
Tanoi, Keitaro

× Tanoi, Keitaro

WEKO 105955

Tanoi, Keitaro

Search repository
Nakanishi, Tomoko M.

× Nakanishi, Tomoko M.

WEKO 105956

Nakanishi, Tomoko M.

Search repository
著者別名
識別子
識別子 105957
識別子Scheme WEKO
姓名
姓名 西山, 宏樹
著者別名
識別子
識別子 105958
識別子Scheme WEKO
姓名
姓名 大矢, 智幸
著者別名
識別子
識別子 105959
識別子Scheme WEKO
姓名
姓名 田野井, 慶太朗
著者別名
識別子
識別子 105960
識別子Scheme WEKO
姓名
姓名 中西, 友子
抄録
内容記述タイプ Abstract
内容記述 We present a simple procedure for measuring the pH in the apoplast of the rice root, using the fluorescent ratio method and Oregon Green 488 (OG). Staining with 5 μM OG for 3 min followed by 5-min washing with calcium solution was an effective method for measuring the pH of the apoplast at the root surface. In order to prevent the diffusion of OG from the apoplast, the circulation of a low concentration of OG (0.02 μM) in a root vessel was found to be a useful modality. However, aluminum ion disturbed pH measure-ment. In the presence of 50 μM AlCl3, the pH value was inaccurate at the pH rage higher than pH 5.0.
書誌情報 Plant Root

巻 2, p. 3-6, 発行日 2008
ISSN
収録物識別子タイプ ISSN
収録物識別子 18816754
DOI
関連識別子
識別子タイプ DOI
関連識別子 info:doi/10.3117/plantroot.2.3
フォーマット
内容記述タイプ Other
内容記述 application/pdf
日本十進分類法
主題 471.1
主題Scheme NDC
出版者
出版者 Japanese Society for Root Research
出版者別名
根研究会
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