Department of Biological Sciences, Graduate School of Science, University of Tokyo
Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo
Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University
Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University
Institute for Advanced Biosciences, Keio University
CREST, Japan Science and Technology Corporation
Division of integrated Omics, Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University
PRESTO, Japan Science and Technology Corporation
抄録
Cellular homeostasis is regulated by signals through multiple molecular networks that include protein phosphorylation and metabolites. However, where and when the signal flows through a network and regulates homeostasis has not been explored. We have developed a reconstruction method for the signal flow based on time-course phosphoproteome and metabolome data, using multiple databases, and have applied it to acute action of insulin, an important hormone for metabolic homeostasis. An insulin signal flows through a network, through signaling pathways that involve 13 protein kinases, 26 phosphorylated metabolic enzymes, and 35 allosteric effectors, resulting in quantitative changes in 44 metabolites. Analysis of the network reveals that insulin induces phosphorylation and activation of liver-type phosphofructokinase 1, thereby controlling a key reaction in glycolysis. We thus provide a versatile method of reconstruction of signal flow through the network using phosphoproteome and metabolome data.
内容記述
UTokyo Research掲載「細胞内のビッグデータから大規模ネットワークの再構築に成功」URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/reconstruction-of-molecular-network-from-cellular-big-data/
UTokyo Research "Reconstruction of molecular network from cellular big data" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/reconstruction-of-molecular-network-from-cellular-big-data/
Reconstruction of Insulin Signal Flow from Phosphoproteome and Metabolome Data
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