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Immunohistochemical identification of hyperglycemic hormone- and molt-inhibiting hormone-producing cells in the eyestalk of the kuruma prawn, Penaeus japonicus
http://hdl.handle.net/2261/8070
http://hdl.handle.net/2261/80704adf79e3-eb87-4e16-8bc8-1f112ab91bea
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ZS15_389.pdf (854.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-12-14 | |||||
| タイトル | ||||||
| タイトル | Immunohistochemical identification of hyperglycemic hormone- and molt-inhibiting hormone-producing cells in the eyestalk of the kuruma prawn, Penaeus japonicus | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Shih, Tung-Wei
× Shih, Tung-Wei× Suzuki, Yuzuru× Nagasawa, Hiromichi× Aida, Katsumi |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 105882 | |||||
| 姓名 | 長澤, 寛道 | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo | |||||
| 著者所属 | ||||||
| 著者所属 | Ocean Research Institute, The University of Tokyo | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This study deals with the localization of crustacean hyperglycemic hormone (CHH, Pej-SGP-III) and molt-inhibiting hormone (MIH, Pej-SGP-IV) in the eyestalk of the kuruma prawn Penaeus japonicus using immunohistochemistry. High-titer and highly specific antisera were raised in rabbits against synthetic Pej-SGP-III C-terminal peptide (Glu-Glu-His-Met-Ala-Ala-Met-Gln-Thr-Val-NH2) and Pej-SGP-IV C-terminal peptide (Val-Trp-Ile-Ser-Ile-Leu-Asn-Ala-Gly-Gln-OH), both of which were conjugated with bovine serum albumin by a cross linker. Eyestalks were removed from mature male prawns at the intermolt stage of the molting cycle and fixed in Bouin's solution. Serial sections stained immunohistochemically showed that neurosecretory cells of Pej-SGP-III and Pej-SGP-IV were located in the same cluster of the medulla terminalis ganglionic X-organ (MTGX), and that three kinds of neurosecretory cells, which were stained with anti-Pej-SGP-III antiserum and/or anti-Pej-SGP-IV antiserum were present. The number of neurosecretory cells which stained with both antisera was much fewer than that of neurosecretory cells which stained with one of the antisera only. The axon and axon terminals in the sinus gland were also stained and the staining density of the sinus gland was always deeper than that of the neurosecretory cells. | |||||
| 書誌情報 |
Zoological science 巻 15, 号 3, p. 389-397, 発行日 1998 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 02890003 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10545874 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.2108/zsj.15.389 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 480 | |||||
| 出版者 | ||||||
| 出版者 | Zoological Society of Japan | |||||
| 出版者別名 | ||||||
| 日本動物学会 | ||||||
