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Cloning and expression of a cDNA encoding an insoluble matrix protein in the gastroliths of a crayfish, Procambarus clarkii
http://hdl.handle.net/2261/8073
http://hdl.handle.net/2261/8073d456fd06-85d0-4ed3-aefa-4cc05f2a63de
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ZS16_619.pdf (504.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-12-14 | |||||
| タイトル | ||||||
| タイトル | Cloning and expression of a cDNA encoding an insoluble matrix protein in the gastroliths of a crayfish, Procambarus clarkii | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Tsutsui, Naoaki
× Tsutsui, Naoaki× Ishii, Katsuaki× Takagi, Yasuaki× Watanabe, Toshiki× Nagasawa, Hiromichi |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 105894 | |||||
| 姓名 | 長澤, 寛道 | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Molecular Biology, Ocean Research Institute, The University of Tokyo | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Applied Biological Chemistry, The University of Tokyo | |||||
| 著者所属 | ||||||
| 著者所属 | Otsuchi Marine Research Center, Ocean Research Institute, The University of Tokyo | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In the crayfish Procambarus clarkii, the gastroliths are formed as a paired structure in the stomach during the premolt period, and contain calcium carbonate and a small amount of an organic matrix. In this investigation, a cDNA encoding an insoluble matrix protein was isolated from P. clarkii. The open reading frame encoded 505 amino acid residues including two unique repeated sequences. The N-terminal half of the amino acid sequence, which included 10-amino-acid repeats, exhibited a high degree of similarity to that of involucrin, a protein synthesized in human keratinocytes. Northern blot analysis revealed that mRNA encoding the matrix protein is specifically expressed in the gastrolith discs during the premolt period in which the gastroliths formed. In the gastrolith discs, levels of expression of this mRNA correlated increases in weights of the gastroliths concomitant with their formation. Organ culture of the gastrolith discs suggested that expression of mRNA in the discs is induced by molting hormone, 20-hydroxyecdysone. These results reinforced the relationship between the matrix protein and formation of the gastroliths. Functional analysis showed that the protein inhibits calcium carbonate crystallization in a solution system, suggesting that the protein plays a role in the calcification of the gastroliths. | |||||
| 書誌情報 |
Zoological science 巻 16, 号 4, p. 619-628, 発行日 1999 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 02890003 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10545874 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.2108/zsj.16.619 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 480 | |||||
| 出版者 | ||||||
| 出版者 | Zoological Society of Japan | |||||
| 出版者別名 | ||||||
| 日本動物学会 | ||||||
