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アイテム
Construction of Mechanical Property Measurement System of Sensor Protein Msb2p and Hkr1p Using Atomic Force Microscopy
http://hdl.handle.net/2261/48927
http://hdl.handle.net/2261/4892707975c0d-b687-40ef-8e26-ce014cebd90a
| 名前 / ファイル | ライセンス | アクション |
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本文(fulltext) (739.5 kB)
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要旨(summary) (256.6 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-10-12 | |||||
| タイトル | ||||||
| タイトル | Construction of Mechanical Property Measurement System of Sensor Protein Msb2p and Hkr1p Using Atomic Force Microscopy | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 出芽酵母 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 原子間力顕微鏡 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | センサータンパク質 | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | 原子間力顕微鏡を用いたセンサータンパクMsb2pとHkr1pの機械的性質測定系の構築 | |||||
| 著者 |
Hiramoto, Ryusuke
× Hiramoto, Ryusuke |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 8782 | |||||
| 姓名 | 平本, 隆輔 | |||||
| 著者所属 | ||||||
| 著者所属 | 東京大学大学院新領域創成科学研究科生命科学研究系先端生命科学専攻 | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo | |||||
| Abstract | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In the yeast Saccharomyces cerevisiae, remodeling of cellular function is required for the response to extracellular stresses. This response is initiated when a sensor protein spanning the plasma membrane perceives stresses. However, its sensing mechanism towards variety of stresses is obscure. To obtain the details on the mechanism and function of a sensor protein, Dufrêne and his co-workers established a method to evaluate the mechanical properties of sensor proteins using the atomic force microscopy (AFM), and clarified that Wsc1p, a sensor protein of cell wall integrity pathway, behaves like a Hookean nanospring. It is possible that the Wsc1p perceives the tension of the glucan chain caused by stresses such as hypo-osmotic shock. The study further revealed that glycosylated Wsc1p perceives the mechanical tension enforced on the cell wall through its linkage with the glucan chain of the call wall. In hyperosmotic condition, cell wall is also subjected to change in its tension due to the rapid shift in turgor pressure. Thus, sensing mechanism of hyperosmotic environment is likely to have a similar property to Wsc1p, however, the difference and similarity of their sensing mechanisms were never investigated. Here I focused on Msb2p and Hkr1p, putative sensor proteins of high osmolarity glycerol (HOG) pathway which is activated by hyperosmotic shock, to clarify the nature of the sensor proteins and its sensing mechanism. In this study, I prepared the construct suitable for AFM analysis. The modified Msb2p was confirmed its function and existence in mother cell surface. The construction and confirmation of the fusion protein is essential for the further study using AFM. | |||||
| 書誌情報 | 発行日 2011-03-24 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 464 | |||||
| 学位名 | ||||||
| 学位名 | 修士(生命科学) | |||||
| 学位 | ||||||
| 値 | master | |||||
| 研究科・専攻 | ||||||
| 新領域創成科学研究科生命科学研究系先端生命科学専攻 | ||||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2011-03-24 | |||||
| 学位記番号 | ||||||
| 修創域第3801号 | ||||||
