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Theoretical Analysis of α-Actin Stability at High Pressure
http://hdl.handle.net/2261/49108
http://hdl.handle.net/2261/49108ed0d2891-78e5-472a-ac50-1c9578c8ec01
| 名前 / ファイル | ライセンス | アクション |
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本文(fulltext) (5.7 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-10-12 | |||||
| タイトル | ||||||
| タイトル | Theoretical Analysis of α-Actin Stability at High Pressure | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | actin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | molecular dynamics | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | high pressure | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | deep-sea fish | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| 著者 |
Wakai, Nobuhiko
× Wakai, Nobuhiko |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 9270 | |||||
| 姓名 | 若井, 信彦 | |||||
| 著者所属 | ||||||
| 著者所属 | 東京大学大学院新領域創成科学研究科情報生命科学専攻 | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo | |||||
| Abstract | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The main component of muscle fiber is α-actin, which plays many important roles in cellular functions. Monomeric actin protein can polymerize into filament with ATP hydrolysis. The amino acid sequences of actins are known to be highly conserved in many species. Deep-sea fish actins have the specific substitutions, Q137K/V54A or Q137K/L67P. Since only Q137K substitution is located near the active site, this residue is expected to contribute to the pressure tolerance. Although some experiments showed the effects of substitutions at high pressure, the detailed mechanism of pressure tolerance induced by substitutions is still unclear, especially in molecular level. Using the molecular dynamics simulations of various actins, I analyzed high pressure effects on actin structures and dynamics. In deep-sea fish actins, the number of salt bridges increases and several salt bridges specific to deep-sea fish actins are observed. The salt bridge between Lys-137 and ATP in deep-sea fish actins stabilizes ATP binding at the active site under high pressure. Other deep-sea specific salt bridges mostly connect pairs of secondary structures and subdomains. The salt bridges which deep-sea fish have are suggested to be a key for the mechanism of the pressure tolerance. | |||||
| 書誌情報 | 発行日 2011-03-24 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 464 | |||||
| 学位名 | ||||||
| 学位名 | 修士(科学) | |||||
| 学位 | ||||||
| 値 | master | |||||
| 研究科・専攻 | ||||||
| 新領域創成科学研究科情報生命科学専攻 | ||||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2011-03-24 | |||||
| 学位記番号 | ||||||
| 修創域第4071号 | ||||||
