{"_buckets": {"deposit": "dcbb5495-9363-4f57-b287-1b81fc0d9bbd"}, "_deposit": {"id": "4009", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "4009"}, "status": "published"}, "_oai": {"id": "oai:repository.dl.itc.u-tokyo.ac.jp:00004009"}, "item_7_biblio_info_7": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-03-24", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_7_date_granted_25": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5", "attribute_value_mlt": [{"subitem_dategranted": "2011-03-24"}]}, "item_7_degree_name_20": {"attribute_name": "\u5b66\u4f4d\u540d", "attribute_value_mlt": [{"subitem_degreename": "\u4fee\u58eb(\u79d1\u5b66)"}]}, "item_7_description_5": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The main component of muscle fiber is \u03b1-actin, which plays many important roles in cellular functions. Monomeric actin protein can polymerize into filament with ATP hydrolysis. The amino acid sequences of actins are known to be highly conserved in many species. Deep-sea fish actins have the specific substitutions, Q137K/V54A or Q137K/L67P. Since only Q137K substitution is located near the active site, this residue is expected to contribute to the pressure tolerance. Although some experiments showed the effects of substitutions at high pressure, the detailed mechanism of pressure tolerance induced by substitutions is still unclear, especially in molecular level. Using the molecular dynamics simulations of various actins, I analyzed high pressure effects on actin structures and dynamics. In deep-sea fish actins, the number of salt bridges increases and several salt bridges specific to deep-sea fish actins are observed. The salt bridge between Lys-137 and ATP in deep-sea fish actins stabilizes ATP binding at the active site under high pressure. Other deep-sea specific salt bridges mostly connect pairs of secondary structures and subdomains. The salt bridges which deep-sea fish have are suggested to be a key for the mechanism of the pressure tolerance.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "9270", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u82e5\u4e95, \u4fe1\u5f66"}]}]}, "item_7_select_21": {"attribute_name": "\u5b66\u4f4d", "attribute_value_mlt": [{"subitem_select_item": "master"}]}, "item_7_subject_13": {"attribute_name": "\u65e5\u672c\u5341\u9032\u5206\u985e\u6cd5", "attribute_value_mlt": [{"subitem_subject": "464", "subitem_subject_scheme": "NDC"}]}, "item_7_text_24": {"attribute_name": "\u7814\u7a76\u79d1\u30fb\u5c02\u653b", "attribute_value_mlt": [{"subitem_text_value": "\u65b0\u9818\u57df\u5275\u6210\u79d1\u5b66\u7814\u7a76\u79d1\u60c5\u5831\u751f\u547d\u79d1\u5b66\u5c02\u653b"}]}, "item_7_text_27": {"attribute_name": "\u5b66\u4f4d\u8a18\u756a\u53f7", "attribute_value_mlt": [{"subitem_text_value": "\u4fee\u5275\u57df\u7b2c4071\u53f7"}]}, "item_7_text_36": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_text_value": "Thesis"}]}, "item_7_text_4": {"attribute_name": "\u8457\u8005\u6240\u5c5e", "attribute_value_mlt": [{"subitem_text_value": "\u6771\u4eac\u5927\u5b66\u5927\u5b66\u9662\u65b0\u9818\u57df\u5275\u6210\u79d1\u5b66\u7814\u7a76\u79d1\u60c5\u5831\u751f\u547d\u79d1\u5b66\u5c02\u653b"}, {"subitem_text_value": "Department of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Wakai, Nobuhiko"}], "nameIdentifiers": [{"nameIdentifier": "9269", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-06-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "K-02949.pdf", "filesize": [{"value": "5.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 5700000.0, "url": {"label": "\u672c\u6587(fulltext)", "url": "https://repository.dl.itc.u-tokyo.ac.jp/record/4009/files/K-02949.pdf"}, "version_id": "3a3d7c0e-df83-41aa-9ef9-a8562492acf8"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-06-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "K-02949-a.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1000000.0, "url": {"label": "\u8981\u65e8(summary)", "url": "https://repository.dl.itc.u-tokyo.ac.jp/record/4009/files/K-02949-a.pdf"}, "version_id": "50b4b917-56f8-461a-a47f-99dd4f74b4c0"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "actin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "molecular dynamics", "subitem_subject_scheme": "Other"}, {"subitem_subject": "high pressure", "subitem_subject_scheme": "Other"}, {"subitem_subject": "deep-sea fish", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Theoretical Analysis of \u03b1-Actin Stability at High Pressure", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Theoretical Analysis of \u03b1-Actin Stability at High Pressure"}]}, "item_type_id": "7", "owner": "1", "path": ["9/233/234", "110/246/247"], "permalink_uri": "http://hdl.handle.net/2261/49108", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2012-10-12"}, "publish_date": "2012-10-12", "publish_status": "0", "recid": "4009", "relation": {}, "relation_version_is_last": true, "title": ["Theoretical Analysis of \u03b1-Actin Stability at High Pressure"], "weko_shared_id": null}