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Mitochondrial gene expression system involving extensive frameshift in a shellfish pathogen Perkinsus
https://doi.org/10.15083/00004116
https://doi.org/10.15083/00004116ad2764a5-e636-4a93-b7f0-11c00e45abec
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
h22_masuda.pdf (2.8 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-11-12 | |||||
| タイトル | ||||||
| タイトル | Mitochondrial gene expression system involving extensive frameshift in a shellfish pathogen Perkinsus | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| ID登録 | ||||||
| ID登録 | 10.15083/00004116 | |||||
| ID登録タイプ | JaLC | |||||
| その他のタイトル | ||||||
| その他のタイトル | 貝類寄生虫Perkinsusにおける高頻度なフレームシフトを伴うミトコンドリア遺伝子発現系 | |||||
| 著者 |
Masuda, Isao
× Masuda, Isao |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 9486 | |||||
| 姓名 | 増田, 功 | |||||
| 著者所属 | ||||||
| 著者所属 | 東京大学大学院医学系研究科国際保健学専攻 | |||||
| Abstract | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Apicomplexan parasites are the causative agents of human infectious diseases including malaria. Modified metabolic pathways and unique gene expression systems of the parasites'mitochondria are promising drug targets. From an evolutionary viewpoint, in this study, I investigated mitochondria of Perkinsus, a relative of apicomplexans. The enrichment method for Perkinsus mitochondria was preliminarily established using Percoll density gradient ultracentrifugation. Perkinsus cells obviously showed enzyme activity ensuring the presence of the active mitochondrial electron-transport chain, and homologs of major subunits of mitochondrial electron-transport chain proteins of P. marinus were found from a public database. Although the sequence searches did not detect possible Perkinsus mitochondrial genes, I could determine the full-length mRNA sequence of cox1. Similar to apicomplexans, this gene lacked canonical start and stop codons in terminal regions. Curiously, this mRNA was not translated in a single reading frame with standard codon usage. Careful examination of the nucleotide sequence and its three-frame translation suggested that the reading frame must be shifted 10 times, at every AGG and CCC codon to yield a consensus COX1 protein. Two possible mechanisms were proposed for the frameshifts: ribosomal frameshifts in which stalled ribosomes skip the first bases of these codons or specialized tRNAs recognizing non-triplet codons, AGGY and CCCCU. Notably, this is the most extensive frameshift case described to date, which would utilize active and efficient machinery. It is valuable that this is a fundamental study on Perkinsus mitochondria and would provide evolutionary insights into mitochondrial functions of Perkinsus and their relatives including apicomplexans. | |||||
| 書誌情報 | 発行日 2011-03-24 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 490 | |||||
| 学位名 | ||||||
| 学位名 | 博士(保健学) | |||||
| 学位 | ||||||
| 値 | doctoral | |||||
| 学位分野 | ||||||
| Health Science (保健学) | ||||||
| 学位授与機関 | ||||||
| 学位授与機関名 | University of Tokyo (東京大学) | |||||
| 研究科・専攻 | ||||||
| Department of International Health, Graduate School of Medicine (医学系研究科国際保健学専攻) | ||||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2011-03-24 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第27141号 | |||||
| 学位記番号 | ||||||
| 博医第3751号 | ||||||
