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  1. 112 医学系研究科・医学部
  2. 22 国際保健学専攻
  3. 1122220 博士論文(国際保健学専攻)
  1. 0 資料タイプ別
  2. 20 学位論文
  3. 021 博士論文

Mitochondrial gene expression system involving extensive frameshift in a shellfish pathogen Perkinsus

https://doi.org/10.15083/00004116
https://doi.org/10.15083/00004116
ad2764a5-e636-4a93-b7f0-11c00e45abec
名前 / ファイル ライセンス アクション
h22_masuda.pdf h22_masuda.pdf (2.8 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2012-11-12
タイトル
タイトル Mitochondrial gene expression system involving extensive frameshift in a shellfish pathogen Perkinsus
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
ID登録
ID登録 10.15083/00004116
ID登録タイプ JaLC
その他のタイトル
その他のタイトル 貝類寄生虫Perkinsusにおける高頻度なフレームシフトを伴うミトコンドリア遺伝子発現系
著者 Masuda, Isao

× Masuda, Isao

WEKO 9485

Masuda, Isao

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著者別名
識別子Scheme WEKO
識別子 9486
姓名 増田, 功
著者所属
著者所属 東京大学大学院医学系研究科国際保健学専攻
Abstract
内容記述タイプ Abstract
内容記述 Apicomplexan parasites are the causative agents of human infectious diseases including malaria. Modified metabolic pathways and unique gene expression systems of the parasites'mitochondria are promising drug targets. From an evolutionary viewpoint, in this study, I investigated mitochondria of Perkinsus, a relative of apicomplexans. The enrichment method for Perkinsus mitochondria was preliminarily established using Percoll density gradient ultracentrifugation. Perkinsus cells obviously showed enzyme activity ensuring the presence of the active mitochondrial electron-transport chain, and homologs of major subunits of mitochondrial electron-transport chain proteins of P. marinus were found from a public database. Although the sequence searches did not detect possible Perkinsus mitochondrial genes, I could determine the full-length mRNA sequence of cox1. Similar to apicomplexans, this gene lacked canonical start and stop codons in terminal regions. Curiously, this mRNA was not translated in a single reading frame with standard codon usage. Careful examination of the nucleotide sequence and its three-frame translation suggested that the reading frame must be shifted 10 times, at every AGG and CCC codon to yield a consensus COX1 protein. Two possible mechanisms were proposed for the frameshifts: ribosomal frameshifts in which stalled ribosomes skip the first bases of these codons or specialized tRNAs recognizing non-triplet codons, AGGY and CCCCU. Notably, this is the most extensive frameshift case described to date, which would utilize active and efficient machinery. It is valuable that this is a fundamental study on Perkinsus mitochondria and would provide evolutionary insights into mitochondrial functions of Perkinsus and their relatives including apicomplexans.
書誌情報 発行日 2011-03-24
日本十進分類法
主題Scheme NDC
主題 490
学位名
学位名 博士(保健学)
学位
値 doctoral
学位分野
Health Science (保健学)
学位授与機関
学位授与機関名 University of Tokyo (東京大学)
研究科・専攻
Department of International Health, Graduate School of Medicine (医学系研究科国際保健学専攻)
学位授与年月日
学位授与年月日 2011-03-24
学位授与番号
学位授与番号 甲第27141号
学位記番号
博医第3751号
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