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Purification, crystallization and preliminary X-ray crystallographic analysis of the human heat-shock protein 40 Hdj1 and its C-terminal peptide-binding domain
http://hdl.handle.net/2261/39052
http://hdl.handle.net/2261/390520afff484-4da3-43e1-90be-2461a2be0c69
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2010_Hdj1_crystallization_ActaF.pdf (13.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2010-12-07 | |||||
| タイトル | ||||||
| タイトル | Purification, crystallization and preliminary X-ray crystallographic analysis of the human heat-shock protein 40 Hdj1 and its C-terminal peptide-binding domain | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Suzuki, Hironori
× Suzuki, Hironori× Noguchi, Shuji× Arakawa, Hiroshi× Tokida, Tadaaki× Hashimoto, Mariko× Satow, Yoshinori |
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| 著者所属 | ||||||
| 著者所属 | Graduate School of Pharmaceutical Sciences, The University of Tokyo | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Hsp40 is a co-chaperone of Hsp70 that correctly folds polypeptides that exist in non-native forms. The C-terminal peptide-binding domain (CTD) of the human Hsp40 Hdj1 has been purified and crystallized. In the presence of the C-terminal octapeptide of human Hsp70, four types of crystals, types I-B, II, III and IV, were grown and diffracted to 1.85, 2.51, 2.10 and 2.80 A ˚ resolution, respectively. In the absence of the octapeptide, type I-A crystals of the CTD were grown that diffracted to 2.05 A ˚ resolution. The full-length Hdj1 was also purified and crystallized (type V crystals); the crystal diffracted to 3.90 A ˚ resolution. | |||||
| bibliographic_information |
Acta crystallographica. Section F, Structural biology and crystallization communications 巻 66, 号 12, p. 1591-1595, 発行日 2010-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 17443091 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12097708 | |||||
| item_2_relation_11 | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1107/S1744309110034081 | |||||
| 権利 | ||||||
| 権利情報 | International Union of Crystallography | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 499 | |||||
| 出版者 | ||||||
| 出版者 | International Union of Crystallography | |||||
