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Purification, crystallization and preliminary X-ray crystallographic analysis of the human heat-shock protein 40 Hdj1 and its C-terminal peptide-binding domain
http://hdl.handle.net/2261/39052
http://hdl.handle.net/2261/390520afff484-4da3-43e1-90be-2461a2be0c69
名前 / ファイル | ライセンス | アクション |
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2010_Hdj1_crystallization_ActaF.pdf (13.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-12-07 | |||||
タイトル | ||||||
タイトル | Purification, crystallization and preliminary X-ray crystallographic analysis of the human heat-shock protein 40 Hdj1 and its C-terminal peptide-binding domain | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Suzuki, Hironori
× Suzuki, Hironori× Noguchi, Shuji× Arakawa, Hiroshi× Tokida, Tadaaki× Hashimoto, Mariko× Satow, Yoshinori |
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著者所属 | ||||||
値 | Graduate School of Pharmaceutical Sciences, The University of Tokyo | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Hsp40 is a co-chaperone of Hsp70 that correctly folds polypeptides that exist in non-native forms. The C-terminal peptide-binding domain (CTD) of the human Hsp40 Hdj1 has been purified and crystallized. In the presence of the C-terminal octapeptide of human Hsp70, four types of crystals, types I-B, II, III and IV, were grown and diffracted to 1.85, 2.51, 2.10 and 2.80 A ˚ resolution, respectively. In the absence of the octapeptide, type I-A crystals of the CTD were grown that diffracted to 2.05 A ˚ resolution. The full-length Hdj1 was also purified and crystallized (type V crystals); the crystal diffracted to 3.90 A ˚ resolution. | |||||
書誌情報 |
Acta crystallographica. Section F, Structural biology and crystallization communications 巻 66, 号 12, p. 1591-1595, 発行日 2010-11 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 17443091 | |||||
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収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12097708 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1107/S1744309110034081 | |||||
権利 | ||||||
権利情報 | International Union of Crystallography | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 499 | |||||
出版者 | ||||||
出版者 | International Union of Crystallography |