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A simple method for identifying two species, Phoca largha and P. vitulina, based on mitochondrial DNA suggests their possible hybridization around Hokkaido, Japan
https://doi.org/10.15083/00079481
https://doi.org/10.15083/000794814b517ed5-5909-4ef4-917c-a94d19e2a3d0
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
CMS430102.pdf (1.6 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-08-31 | |||||
| タイトル | ||||||
| タイトル | A simple method for identifying two species, Phoca largha and P. vitulina, based on mitochondrial DNA suggests their possible hybridization around Hokkaido, Japan | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PCR-RFLP | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | seals | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | species identification | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | natural hybridization | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.15083/00079481 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
Azuma, Noriko
× Azuma, Noriko× Kobayashi, Nozomi× Kikkawa, Yoshiaki× Kobayashi, Mari |
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| 著者所属 | ||||||
| 著者所属 | Marine Wildlife Center of Japan | |||||
| 著者所属 | ||||||
| 著者所属 | Faculty of Bioindustry, Tokyo University of Agriculture | |||||
| 著者所属 | ||||||
| 著者所属 | Mammalian Genetics Project, Tokyo Metropolitan Institute of Medical Science | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have developed a method for identifying maternal lineage of two species, the spotted seal Phoca largha and the harbor seal P. vitulina, using restriction fragment length polymorphism of PCR products (PCR-RFLP) in mitochondrial DNA. A partial sequence of the cytochrome b gene (Cytb) was amplified and digested with AluI in both species. In agarose gel electrophoresis digested PCR product from P. largha showed two bands, while one from P. vitulina showed a single band. Sequence analysis confirmed the precise detection of mitochondrial DNA lineage using PCR-RFLP methods developed in the present study. Some exceptions, where morphological character, color and pattern of fur, and mtDNA type were inconsistent, were also observed, suggesting possible hybridization of these species, as well as the possibility of misidentification by fur color and pattern. | |||||
| 書誌情報 |
Coastal marine science 巻 43, 号 1, p. 26-31, 発行日 2020 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13493000 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11957899 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| 出版者 | ||||||
| 出版者 | International Coastal Research Center, Atmosphere and Ocean Research Institute, the University of Tokyo | |||||
| 出版者別名 | ||||||
| 東京大学大気海洋研究所国際沿岸海洋研究センター | ||||||
