{"_buckets": {"deposit": "ce04de84-5ba0-4502-9cd0-253c7e66aa60"}, "_deposit": {"id": "697", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "697"}, "status": "published"}, "_oai": {"id": "oai:repository.dl.itc.u-tokyo.ac.jp:00000697"}, "item_2_biblio_info_7": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-07-04", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "19163", "bibliographicPageStart": "19150", "bibliographicVolumeNumber": "289", "bibliographic_titles": [{"bibliographic_title": "The journal of biological chemistry"}]}]}, "item_2_description_5": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The biosynthesis of sex pheromone components in many lepidopteran insects is regulated by the interaction between pheromone biosynthesis-activating neuropeptide (PBAN) and the PBAN receptor (PBANR), a class A G-protein-coupled receptor. To identify functionally important amino acid residues in the silkmoth PBANR, a series of 27 alanine substitutions was generated using a PBANR chimera C-terminally fused with enhanced GFP. The PBANR mutants were expressed in Sf9 insect cells, and their ability to bind and be activated by a core PBAN fragment (C10PBANR2K) was monitored. Among the 27 mutants, 23 localized to the cell surface of transfected Sf9 cells, whereas the other four remained intracellular. Reduced binding relative to wild type was observed with 17 mutants, and decreased Ca2+ mobilization responses were observed with 12 mutants. Ala substitution of Glu-95, Glu-120, Asn-124, Val-195, Phe-276, Trp-280, Phe-283, Arg-287, Tyr-307, Thr-311, and Phe-319 affected both binding and Ca2+ mobilization. The most pronounced effects were observed with the E120A mutation. A molecular model of PBANR indicated that the functionally important PBANR residues map to the 2nd, 3rd, 6th, and 7th transmembrane helices, implying that the same general region of class A G-protein-coupled receptors recognizes both peptidic and nonpeptidic ligands. Docking simulations suggest similar ligand-receptor recognition interactions for PBAN-PBANR and the orthologous vertebrate pair, neuromedin U (NMU) and NMU receptor (NMUR). The simulations highlight the importance of two glutamate residues, Glu-95 and Glu-120, in silkmoth PBANR and Glu-117 and Glu-142 in human NMUR1, in the recognition of the most functionally critical region of the ligands, the C-terminal residue and amide.", "subitem_description_type": "Abstract"}]}, "item_2_description_6": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "UTokyo Research\u63b2\u8f09\u300c\u86fe\u306e\u6027\u30d5\u30a7\u30ed\u30e2\u30f3\u751f\u5408\u6210\u3068\u4eba\u306e\u30b9\u30c8\u30ec\u30b9\u30fb\u98df\u6b32\u5236\u5fa1\u306e\u985e\u4f3c\u6027\u300d URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/similarities-in-moth-pheromone-synthesis-and-human-stress-appetite-control/", "subitem_description_type": "Other"}, {"subitem_description": "UTokyo Research: \"Similarities in moth pheromone synthesis and human stress/appetite control\" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/similarities-in-moth-pheromone-synthesis-and-human-stress-appetite-control/", "subitem_description_type": "Other"}]}, "item_2_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "2558", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u6cb3\u5408, \u5cb3\u5fd7"}]}, {"nameIdentifiers": [{"nameIdentifier": "2559", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u7247\u5c71, \u5e78\u6c5f"}]}, {"nameIdentifiers": [{"nameIdentifier": "2560", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u90ed, \u7433\u73fa"}]}, {"nameIdentifiers": [{"nameIdentifier": "2561", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u5218, \u5fb7\u751f"}]}, {"nameIdentifiers": [{"nameIdentifier": "2562", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u9234\u6728, \u9054\u4e5f"}]}, {"nameIdentifiers": [{"nameIdentifier": "2563", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u65e9\u5ddd, \u6c5f"}]}, {"nameIdentifiers": [{"nameIdentifier": "2564", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u674e, \u8f09\u65fc"}]}, {"nameIdentifiers": [{"nameIdentifier": "2565", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u6c38\u5cf0, \u4fca\u5f18"}]}, {"nameIdentifiers": [{"nameIdentifier": "2566", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u677e\u672c, \u6b63\u543e"}]}, {"nameIdentifiers": [{"nameIdentifier": "2567", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u9577\u6fa4, \u5bdb\u9053"}]}, {"nameIdentifiers": [{"nameIdentifier": "2568", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u7530\u4e4b\u5009, \u512a"}]}, {"nameIdentifiers": [{"nameIdentifier": "2569", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u6c38\u7530, \u5b8f\u6b21"}]}]}, "item_2_publisher_20": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "The American Society for Biochemistry and Molecular Biology"}]}, "item_2_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1074/jbc.M113.488999", "subitem_relation_type_select": "DOI"}}]}, "item_2_relation_25": {"attribute_name": "\u95a2\u4fc2URI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/similarities-in-moth-pheromone-synthesis-and-human-stress-appetite-control/", "subitem_relation_type_select": "URI"}}, {"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/similarities-in-moth-pheromone-synthesis-and-human-stress-appetite-control/", "subitem_relation_type_select": "URI"}}, {"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.jbc.org/content/289/27/19150.abstract", "subitem_relation_type_select": "URI"}}]}, "item_2_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "This research was originally published in the Journal of Biological Chemistry. Takeshi Kawai, Yukie Katayama, Linjun Guo, Desheng Liu, Tatsuya Suzuki, Kou Hayakawa, JaeMin Lee, Toshihiro Nagamine, J. Joe Hull, Shogo Matsumoto, Hiromichi Nagasawa, Masaru Tanokura, Koji Nagata. Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor. The Journal of Biological Chemistry. 2014; 289:19150-19163, doi: 10.1074/jbc.M113.488999. \u00a9 the American Society for Biochemistry and Molecular Biology."}]}, "item_2_select_14": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_2_source_id_10": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00251083", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_8": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0021-9258", "subitem_source_identifier_type": "ISSN"}, {"subitem_source_identifier": "1083-351X", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_34": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_text_value": "Jounal Article"}]}, "item_2_text_4": {"attribute_name": "\u8457\u8005\u6240\u5c5e", "attribute_value_mlt": [{"subitem_text_value": "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo"}, {"subitem_text_value": "Molecular Entomology Laboratory, RIKEN Advanced Science Institute"}, {"subitem_text_value": "United States Department of Agriculture-Arid Land Agricultural Research Center"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kawai, Takeshi"}], "nameIdentifiers": [{"nameIdentifier": "2545", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Katayama, Yukie"}], "nameIdentifiers": [{"nameIdentifier": "2546", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Guo, Linjun"}], "nameIdentifiers": [{"nameIdentifier": "2547", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Liu, Desheng"}], "nameIdentifiers": [{"nameIdentifier": "2548", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Tatsuya"}], "nameIdentifiers": [{"nameIdentifier": "2549", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayakawa, Kou"}], "nameIdentifiers": [{"nameIdentifier": "2550", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Lee, Jae Min"}], "nameIdentifiers": [{"nameIdentifier": "2551", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagamine, Toshihiro"}], "nameIdentifiers": [{"nameIdentifier": "2552", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hull, J. Joe"}], "nameIdentifiers": [{"nameIdentifier": "2553", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Shogo"}], "nameIdentifiers": [{"nameIdentifier": "2554", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagasawa, Hiromichi"}], "nameIdentifiers": [{"nameIdentifier": "2555", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanokura, Masaru"}], "nameIdentifiers": [{"nameIdentifier": "2556", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagata, Koji"}], "nameIdentifiers": [{"nameIdentifier": "2557", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-05-30"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "jbc.M113.488999_manuscript.pdf", "filesize": [{"value": "3.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 3300000.0, "url": {"label": "jbc.M113.488999_manuscript.pdf", "url": "https://repository.dl.itc.u-tokyo.ac.jp/record/697/files/jbc.M113.488999_manuscript.pdf"}, "version_id": "a76a7eba-acd4-4e72-880c-db84aa9cc7c3"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "G-protein-coupled Receptor (GPCR)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Molecular Docking", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Molecular Modeling", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Peptide Hormone", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Receptor Structure-Function", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Silkworm", "subitem_subject_scheme": "Other"}, {"subitem_subject": "FXPRX-amide Motif", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ligand-Receptor Interaction", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Neuromedin U", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sex Pheromone Biosynthesis", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor"}]}, "item_type_id": "2", "owner": "1", "path": ["9/10/11", "27/38/39"], "permalink_uri": "http://hdl.handle.net/2261/57057", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2015-07-08"}, "publish_date": "2015-07-08", "publish_status": "0", "recid": "697", "relation": {}, "relation_version_is_last": true, "title": ["Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor"], "weko_shared_id": 2}