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Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor
http://hdl.handle.net/2261/57057
http://hdl.handle.net/2261/5705756181618-c549-4730-958a-9c4534324972
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
jbc.M113.488999_manuscript.pdf (3.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2015-07-08 | |||||
| タイトル | ||||||
| タイトル | Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | G-protein-coupled Receptor (GPCR) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Molecular Docking | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Molecular Modeling | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Peptide Hormone | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Receptor Structure-Function | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Silkworm | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | FXPRX-amide Motif | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ligand-Receptor Interaction | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Neuromedin U | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Sex Pheromone Biosynthesis | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Kawai, Takeshi
× Kawai, Takeshi× Katayama, Yukie× Guo, Linjun× Liu, Desheng× Suzuki, Tatsuya× Hayakawa, Kou× Lee, Jae Min× Nagamine, Toshihiro× Hull, J. Joe× Matsumoto, Shogo× Nagasawa, Hiromichi× Tanokura, Masaru× Nagata, Koji |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2558 | |||||
| 姓名 | 河合, 岳志 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2559 | |||||
| 姓名 | 片山, 幸江 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2560 | |||||
| 姓名 | 郭, 琳珺 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2561 | |||||
| 姓名 | 刘, 德生 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2562 | |||||
| 姓名 | 鈴木, 達也 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2563 | |||||
| 姓名 | 早川, 江 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2564 | |||||
| 姓名 | 李, 載旼 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2565 | |||||
| 姓名 | 永峰, 俊弘 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2566 | |||||
| 姓名 | 松本, 正吾 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2567 | |||||
| 姓名 | 長澤, 寛道 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2568 | |||||
| 姓名 | 田之倉, 優 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 2569 | |||||
| 姓名 | 永田, 宏次 | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo | |||||
| 著者所属 | ||||||
| 著者所属 | Molecular Entomology Laboratory, RIKEN Advanced Science Institute | |||||
| 著者所属 | ||||||
| 著者所属 | United States Department of Agriculture-Arid Land Agricultural Research Center | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The biosynthesis of sex pheromone components in many lepidopteran insects is regulated by the interaction between pheromone biosynthesis-activating neuropeptide (PBAN) and the PBAN receptor (PBANR), a class A G-protein-coupled receptor. To identify functionally important amino acid residues in the silkmoth PBANR, a series of 27 alanine substitutions was generated using a PBANR chimera C-terminally fused with enhanced GFP. The PBANR mutants were expressed in Sf9 insect cells, and their ability to bind and be activated by a core PBAN fragment (C10PBANR2K) was monitored. Among the 27 mutants, 23 localized to the cell surface of transfected Sf9 cells, whereas the other four remained intracellular. Reduced binding relative to wild type was observed with 17 mutants, and decreased Ca2+ mobilization responses were observed with 12 mutants. Ala substitution of Glu-95, Glu-120, Asn-124, Val-195, Phe-276, Trp-280, Phe-283, Arg-287, Tyr-307, Thr-311, and Phe-319 affected both binding and Ca2+ mobilization. The most pronounced effects were observed with the E120A mutation. A molecular model of PBANR indicated that the functionally important PBANR residues map to the 2nd, 3rd, 6th, and 7th transmembrane helices, implying that the same general region of class A G-protein-coupled receptors recognizes both peptidic and nonpeptidic ligands. Docking simulations suggest similar ligand-receptor recognition interactions for PBAN-PBANR and the orthologous vertebrate pair, neuromedin U (NMU) and NMU receptor (NMUR). The simulations highlight the importance of two glutamate residues, Glu-95 and Glu-120, in silkmoth PBANR and Glu-117 and Glu-142 in human NMUR1, in the recognition of the most functionally critical region of the ligands, the C-terminal residue and amide. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | UTokyo Research掲載「蛾の性フェロモン生合成と人のストレス・食欲制御の類似性」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/similarities-in-moth-pheromone-synthesis-and-human-stress-appetite-control/ | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | UTokyo Research: "Similarities in moth pheromone synthesis and human stress/appetite control" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/similarities-in-moth-pheromone-synthesis-and-human-stress-appetite-control/ | |||||
| 書誌情報 |
The journal of biological chemistry 巻 289, p. 19150-19163, 発行日 2014-07-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0021-9258 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1083-351X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00251083 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1074/jbc.M113.488999 | |||||
| 権利 | ||||||
| 権利情報 | This research was originally published in the Journal of Biological Chemistry. Takeshi Kawai, Yukie Katayama, Linjun Guo, Desheng Liu, Tatsuya Suzuki, Kou Hayakawa, JaeMin Lee, Toshihiro Nagamine, J. Joe Hull, Shogo Matsumoto, Hiromichi Nagasawa, Masaru Tanokura, Koji Nagata. Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor. The Journal of Biological Chemistry. 2014; 289:19150-19163, doi: 10.1074/jbc.M113.488999. © the American Society for Biochemistry and Molecular Biology. | |||||
| 著者版フラグ | ||||||
| 値 | author | |||||
| 出版者 | ||||||
| 出版者 | The American Society for Biochemistry and Molecular Biology | |||||
| 関係URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/similarities-in-moth-pheromone-synthesis-and-human-stress-appetite-control/ | |||||
| 関係URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/similarities-in-moth-pheromone-synthesis-and-human-stress-appetite-control/ | |||||
| 関係URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jbc.org/content/289/27/19150.abstract | |||||
