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Flexible Sheet-Type Sensor for Noninvasive Measurement of Cellular Oxygen Metabolism on a Culture Dish
http://hdl.handle.net/2261/59066
http://hdl.handle.net/2261/59066da6b8221-5d56-4b3a-a6bb-1572766392a6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
journal.pone.0143774.pdf (2.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2015-12-25 | |||||
| タイトル | ||||||
| タイトル | Flexible Sheet-Type Sensor for Noninvasive Measurement of Cellular Oxygen Metabolism on a Culture Dish | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Kojima, Mari
× Kojima, Mari× Takehara, Hiroaki× Akagi, Takanori× Shiono, Hirofumi× Ichiki, Takanori |
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| 著者所属 | ||||||
| 著者所属 | Department of Bioengineering, School of Engineering, The University of Tokyo | |||||
| 著者所属 | ||||||
| 著者所属 | Nikon Corporation | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A novel flexible sensor was developed for the noninvasive oxygen metabolism measurement of cultivated cells and tissues. This device is composed of a transparent double-layered polymer sheet of ethylene-vinyl alcohol (EVOH) and poly(dimethylsiloxane) (PDMS) having an array of microhole structures of 90 μm diameter and 50 μm depth on its surface. All the microhole structures were equipped with a 1-μm-thick optical chemical sensing layer of platinum porphyrin-fluoropolymer on their bottom. The three-dimensional microstructures of the sensor were fabricated by a newly developed simple and low-cost production method named self-aligned hot embossing. The device was designed to be attached slightly above the cells cultivated on a dish to form a temporarily closed microspace over the target cells during measurement. Since the change in oxygen concentration is relatively fast in the microcompartmentalized culture medium, a rapid evaluation of the oxygen consumption rate is possible by measuring the phosphorescence lifetime of the platinum porphyrin-fluoropolymer. The combined use of the device and an automated optical measurement system enabled the high-throughput sensing of cellular oxygen consumption (100 points/min). We monitored the oxygen metabolism of the human breast cancer cell line MCF7 on a Petri dish and evaluated the oxygen consumption rate to be 0.72 ± 0.12 fmol/min/cell. Furthermore, to demonstrate the utility of the developed sensing system, we demonstrated the mapping of the oxygen consumption rate of rat brain slices and succeeded in visualizing a clear difference among the layer structures of the hippocampus, i.e., the cornu ammonis (CA1 and CA3) and dentate gyrus (DG). | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | UTokyo Research掲載「載せるだけで細胞の酸素消費量を測定できるシート型センサ」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | UTokyo Research "Flexible sheet-type sensor for measuring cellular oxygen metabolism" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html | |||||
| 書誌情報 |
PLOS ONE Online Edition 巻 10, 号 12, 発行日 2015-12-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1932-6203 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1371/journal.pone.0143774 | |||||
| 権利 | ||||||
| 権利情報 | © 2015 Kojima et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| 出版者 | ||||||
| 出版者 | PLOS | |||||
| 関係URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html | |||||
| 関係URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html | |||||
| 関係URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143774 | |||||
