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  1. 116 農学生命科学研究科・農学部
  2. 18 農学国際専攻
  3. 1161810 学術雑誌論文
  1. 0 資料タイプ別
  2. 10 学術雑誌論文
  3. 014 自然科学

The phytosiderophore efflux transporter TOM2 is involved in metal transport in rice

http://hdl.handle.net/2261/60927
a0f3e4bc-b766-41a3-838f-da7945c7b812
名前 / ファイル ライセンス アクション
jbc.M114.635193.pdf jbc.M114.635193.pdf (4.9 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-01-10
タイトル
タイトル The phytosiderophore efflux transporter TOM2 is involved in metal transport in rice
言語
言語 eng
キーワード
主題 iron
主題Scheme Other
キーワード
主題 nutrition
主題Scheme Other
キーワード
主題 plant molecular biology
主題Scheme Other
キーワード
主題 plant physiology
主題Scheme Other
キーワード
主題 transporter
主題Scheme Other
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Nozoye, Tomoko

× Nozoye, Tomoko

WEKO 3714

Nozoye, Tomoko

Search repository
Nagasaka, Seiji

× Nagasaka, Seiji

WEKO 3715

Nagasaka, Seiji

Search repository
Kobayashi, Takanori

× Kobayashi, Takanori

WEKO 3716

Kobayashi, Takanori

Search repository
Sato, Yuki

× Sato, Yuki

WEKO 3717

Sato, Yuki

Search repository
Uozumi, Nobuyuki

× Uozumi, Nobuyuki

WEKO 3718

Uozumi, Nobuyuki

Search repository
Nakanishi, Hiromi

× Nakanishi, Hiromi

WEKO 3719

Nakanishi, Hiromi

Search repository
Nishizawa, Naoko K.

× Nishizawa, Naoko K.

WEKO 3720

Nishizawa, Naoko K.

Search repository
著者所属
著者所属 Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
著者所属
著者所属 Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
著者所属
著者所属 Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
抄録
内容記述タイプ Abstract
内容記述 Iron (Fe) is an essential metal element for all living organisms. Graminaceous plants produce and secrete mugineic acid family phytosiderophores from their roots to acquire Fe in the soil. Phytosiderophores chelate and solubilize insoluble Fe hydroxide in the soil. Subsequently, plants take up Fe-phytosiderophore complexes through specific transporters on the root cell membrane. Phytosiderophores are also thought to be important for the internal transport of various transition metals including Fe. In the present study, we analyzed TOM2 and TOM3, rice homologs of transporter of mugineic acid family phytosiderophores 1 (TOM1), a crucial efflux transporter directly involved in phytosiderophore secretion into the soil. Transgenic rice analysis using promoter-β-glucuronidase (GUS) revealed that TOM2 was expressed in tissues involved in metal translocation, while TOM3 was expressed only in restricted parts of the plant. Strong TOM2 expression was observed in developing tissues during seed maturation and germination, while TOM3 expression was weak during seed maturation. Transgenic rice in which TOM2 expression was repressed by RNA interference showed growth defects compared to non-transformants and TOM3-repressed rice. Xenopus laevis oocytes expressing TOM2 released 14C-labeled deoxymugineic acid (DMA), the initial phytosiderophore compound in the biosynthetic pathway in rice. In onion epidermal and rice root cells, the TOM2-GFP fusion protein localized to the cell membrane, indicating that the TOM2 protein is a transporter for phytosiderophore efflux to the cell exterior. Our results indicate that TOM2 is involved in the internal transport of DMA, which is required for normal plant growth.
内容記述
内容記述タイプ Other
内容記述 UTokyo Research掲載「植物の生育に必要な金属輸送に関与するタンパク質の発見」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/identification-of-a-crucial-metal-transporter-for-plant-growth.html
内容記述
内容記述タイプ Other
内容記述 UTokyo Research "Identification of a crucial metal transporter for plant growth" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/identification-of-a-crucial-metal-transporter-for-plant-growth.html
書誌情報 The journal of biological chemistry

発行日 2015-10-02
ISSN
収録物識別子タイプ ISSN
収録物識別子 1083351X
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA1202441X
DOI
関連識別子
識別子タイプ DOI
関連識別子 info:doi/10.1074/jbc.M114.635193
権利
権利情報 Copyright 2015, by the American Society for Biochemistry and Molecular Biology
著者版フラグ
値 author
出版者
出版者 The American Society for Biochemistry and Molecular Biology
関係URI
関連識別子
識別子タイプ URI
関連識別子 http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/identification-of-a-crucial-metal-transporter-for-plant-growth.html
関係URI
関連識別子
識別子タイプ URI
関連識別子 http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/identification-of-a-crucial-metal-transporter-for-plant-growth.html
異版である
関連タイプ isVersionOf
関連識別子
識別子タイプ URI
関連識別子 http://doi.org/10.1074/jbc.M114.635193
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