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The phytosiderophore efflux transporter TOM2 is involved in metal transport in rice
http://hdl.handle.net/2261/60927
http://hdl.handle.net/2261/60927a0f3e4bc-b766-41a3-838f-da7945c7b812
名前 / ファイル | ライセンス | アクション |
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jbc.M114.635193.pdf (4.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-01-10 | |||||
タイトル | ||||||
タイトル | The phytosiderophore efflux transporter TOM2 is involved in metal transport in rice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | iron | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | nutrition | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | plant molecular biology | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | plant physiology | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | transporter | |||||
主題Scheme | Other | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Nozoye, Tomoko
× Nozoye, Tomoko× Nagasaka, Seiji× Kobayashi, Takanori× Sato, Yuki× Uozumi, Nobuyuki× Nakanishi, Hiromi× Nishizawa, Naoko K. |
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著者所属 | ||||||
著者所属 | Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo | |||||
著者所属 | ||||||
著者所属 | Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University | |||||
著者所属 | ||||||
著者所属 | Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Iron (Fe) is an essential metal element for all living organisms. Graminaceous plants produce and secrete mugineic acid family phytosiderophores from their roots to acquire Fe in the soil. Phytosiderophores chelate and solubilize insoluble Fe hydroxide in the soil. Subsequently, plants take up Fe-phytosiderophore complexes through specific transporters on the root cell membrane. Phytosiderophores are also thought to be important for the internal transport of various transition metals including Fe. In the present study, we analyzed TOM2 and TOM3, rice homologs of transporter of mugineic acid family phytosiderophores 1 (TOM1), a crucial efflux transporter directly involved in phytosiderophore secretion into the soil. Transgenic rice analysis using promoter-β-glucuronidase (GUS) revealed that TOM2 was expressed in tissues involved in metal translocation, while TOM3 was expressed only in restricted parts of the plant. Strong TOM2 expression was observed in developing tissues during seed maturation and germination, while TOM3 expression was weak during seed maturation. Transgenic rice in which TOM2 expression was repressed by RNA interference showed growth defects compared to non-transformants and TOM3-repressed rice. Xenopus laevis oocytes expressing TOM2 released 14C-labeled deoxymugineic acid (DMA), the initial phytosiderophore compound in the biosynthetic pathway in rice. In onion epidermal and rice root cells, the TOM2-GFP fusion protein localized to the cell membrane, indicating that the TOM2 protein is a transporter for phytosiderophore efflux to the cell exterior. Our results indicate that TOM2 is involved in the internal transport of DMA, which is required for normal plant growth. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | UTokyo Research掲載「植物の生育に必要な金属輸送に関与するタンパク質の発見」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/identification-of-a-crucial-metal-transporter-for-plant-growth.html | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | UTokyo Research "Identification of a crucial metal transporter for plant growth" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/identification-of-a-crucial-metal-transporter-for-plant-growth.html | |||||
書誌情報 |
The journal of biological chemistry 発行日 2015-10-02 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1083351X | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1202441X | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1074/jbc.M114.635193 | |||||
権利 | ||||||
権利情報 | Copyright 2015, by the American Society for Biochemistry and Molecular Biology | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | The American Society for Biochemistry and Molecular Biology | |||||
関係URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/identification-of-a-crucial-metal-transporter-for-plant-growth.html | |||||
関係URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/identification-of-a-crucial-metal-transporter-for-plant-growth.html | |||||
異版である | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://doi.org/10.1074/jbc.M114.635193 |