{"_buckets": {"deposit": "643e9f4a-7471-45ca-8c8b-363d7f841ef8"}, "_deposit": {"id": "170", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "170"}, "status": "published"}, "_oai": {"id": "oai:repository.dl.itc.u-tokyo.ac.jp:00000170"}, "item_2_biblio_info_7": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1998", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "937", "bibliographicPageStart": "931", "bibliographicVolumeNumber": "15", "bibliographic_titles": [{"bibliographic_title": "Zoological science"}]}]}, "item_2_description_13": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_2_description_5": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "We have purified and characterized two molecular forms of insulin from the Brockmann bodies of barfin flounder, Verasper moseri: a normal type of insulin (insulin-I), which consisted of 21 amino acid residues for the A-chain and 30 residues for the B-chain, and a novel type of insulin (insulin-II), which had an extension of two amino acid residues at the N-terminus of the B-chain. The additional two residues at the N-terminus of B-chain of insulin-II were Pyr-Ala which had not yet been reported in vertebrate insulins. Except for these two residues, the amino acid sequence of insulin-II was completely identical with that of insulin-I. Each Brockmann body extract from five individuals contained both insulins, indicating that insulin-I and -II were the products of non-allelic expression. By polymerase chain reaction, only one nucleotide sequence of preproinsulin gene encoding insulin-I and -II was obtained, and the amino acid sequence of A- and B-chains deduced from the nucleotide sequence was identical with that of insulin-I and also insulin-II established by Edman degradation. Furthermore, genomic Southern blot analysis using a part of nucleotide sequence of the preproinsulin gene as a probe showed a single positive band in all cases of genomic DNA digested with each of three restriction endonucleases. These results indicate that insulin-I and -II of barfin flounder arise from a single preproinsulin by proteolytic cleavage at different sites of the signal peptide region.", "subitem_description_type": "Abstract"}]}, "item_2_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "105885", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u9577\u6fa4, \u5bdb\u9053"}]}]}, "item_2_publisher_20": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Zoological Society of Japan"}]}, "item_2_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.2108/zsj.15.931", "subitem_relation_type_select": "DOI"}}]}, "item_2_source_id_10": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10545874", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_8": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "02890003", "subitem_source_identifier_type": "ISSN"}]}, "item_2_subject_15": {"attribute_name": "\u65e5\u672c\u5341\u9032\u5206\u985e\u6cd5", "attribute_value_mlt": [{"subitem_subject": "480", "subitem_subject_scheme": "NDC"}]}, "item_2_text_21": {"attribute_name": "\u51fa\u7248\u8005\u5225\u540d", "attribute_value_mlt": [{"subitem_text_value": "\u65e5\u672c\u52d5\u7269\u5b66\u4f1a"}]}, "item_2_text_34": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_text_value": "Journal Article"}]}, "item_2_text_4": {"attribute_name": "\u8457\u8005\u6240\u5c5e", "attribute_value_mlt": [{"subitem_text_value": "Hokkaido National Fisheries Research Institute"}, {"subitem_text_value": "Department of Applied Biochemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Andoh, Tadashi"}], "nameIdentifiers": [{"nameIdentifier": "105883", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagasawa, Hiromichi"}], "nameIdentifiers": [{"nameIdentifier": "105884", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-06-26"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ZS15_931.pdf", "filesize": [{"value": "867.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 867300.0, "url": {"label": "ZS15_931.pdf", "url": "https://repository.dl.itc.u-tokyo.ac.jp/record/170/files/ZS15_931.pdf"}, "version_id": "fbed1b7d-571b-4918-adb0-2ed61c946609"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Two molecular forms of insulin from barfin flounder, Verasper moseri, are derived from a single gene", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Two molecular forms of insulin from barfin flounder, Verasper moseri, are derived from a single gene"}]}, "item_type_id": "2", "owner": "1", "path": ["9/10/11", "27/38/39"], "permalink_uri": "http://hdl.handle.net/2261/8071", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2007-12-14"}, "publish_date": "2007-12-14", "publish_status": "0", "recid": "170", "relation": {}, "relation_version_is_last": true, "title": ["Two molecular forms of insulin from barfin flounder, Verasper moseri, are derived from a single gene"], "weko_shared_id": null}