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Two molecular forms of insulin from barfin flounder, Verasper moseri, are derived from a single gene
http://hdl.handle.net/2261/8071
http://hdl.handle.net/2261/80716c80b358-2a91-42a9-be31-4fbefb6262ad
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ZS15_931.pdf (867.3 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-12-14 | |||||
| タイトル | ||||||
| タイトル | Two molecular forms of insulin from barfin flounder, Verasper moseri, are derived from a single gene | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Andoh, Tadashi
× Andoh, Tadashi× Nagasawa, Hiromichi |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 105885 | |||||
| 姓名 | 長澤, 寛道 | |||||
| 著者所属 | ||||||
| 著者所属 | Hokkaido National Fisheries Research Institute | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Applied Biochemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have purified and characterized two molecular forms of insulin from the Brockmann bodies of barfin flounder, Verasper moseri: a normal type of insulin (insulin-I), which consisted of 21 amino acid residues for the A-chain and 30 residues for the B-chain, and a novel type of insulin (insulin-II), which had an extension of two amino acid residues at the N-terminus of the B-chain. The additional two residues at the N-terminus of B-chain of insulin-II were Pyr-Ala which had not yet been reported in vertebrate insulins. Except for these two residues, the amino acid sequence of insulin-II was completely identical with that of insulin-I. Each Brockmann body extract from five individuals contained both insulins, indicating that insulin-I and -II were the products of non-allelic expression. By polymerase chain reaction, only one nucleotide sequence of preproinsulin gene encoding insulin-I and -II was obtained, and the amino acid sequence of A- and B-chains deduced from the nucleotide sequence was identical with that of insulin-I and also insulin-II established by Edman degradation. Furthermore, genomic Southern blot analysis using a part of nucleotide sequence of the preproinsulin gene as a probe showed a single positive band in all cases of genomic DNA digested with each of three restriction endonucleases. These results indicate that insulin-I and -II of barfin flounder arise from a single preproinsulin by proteolytic cleavage at different sites of the signal peptide region. | |||||
| bibliographic_information |
Zoological science 巻 15, 号 6, p. 931-937, 発行日 1998 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 02890003 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10545874 | |||||
| item_2_relation_11 | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.2108/zsj.15.931 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 480 | |||||
| 出版者 | ||||||
| 出版者 | Zoological Society of Japan | |||||
| 出版者別名 | ||||||
| 日本動物学会 | ||||||
