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  1. 116 農学生命科学研究科・農学部
  2. 04 応用生命化学専攻
  3. 1160410 学術雑誌論文
  1. 0 資料タイプ別
  2. 10 学術雑誌論文
  3. 014 自然科学

Two molecular forms of insulin from barfin flounder, Verasper moseri, are derived from a single gene

http://hdl.handle.net/2261/8071
http://hdl.handle.net/2261/8071
6c80b358-2a91-42a9-be31-4fbefb6262ad
名前 / ファイル ライセンス アクション
ZS15_931.pdf ZS15_931.pdf (867.3 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-12-14
タイトル
タイトル Two molecular forms of insulin from barfin flounder, Verasper moseri, are derived from a single gene
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Andoh, Tadashi

× Andoh, Tadashi

WEKO 105883

Andoh, Tadashi

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Nagasawa, Hiromichi

× Nagasawa, Hiromichi

WEKO 105884

Nagasawa, Hiromichi

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著者別名
識別子Scheme WEKO
識別子 105885
姓名 長澤, 寛道
著者所属
著者所属 Hokkaido National Fisheries Research Institute
著者所属
著者所属 Department of Applied Biochemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
抄録
内容記述タイプ Abstract
内容記述 We have purified and characterized two molecular forms of insulin from the Brockmann bodies of barfin flounder, Verasper moseri: a normal type of insulin (insulin-I), which consisted of 21 amino acid residues for the A-chain and 30 residues for the B-chain, and a novel type of insulin (insulin-II), which had an extension of two amino acid residues at the N-terminus of the B-chain. The additional two residues at the N-terminus of B-chain of insulin-II were Pyr-Ala which had not yet been reported in vertebrate insulins. Except for these two residues, the amino acid sequence of insulin-II was completely identical with that of insulin-I. Each Brockmann body extract from five individuals contained both insulins, indicating that insulin-I and -II were the products of non-allelic expression. By polymerase chain reaction, only one nucleotide sequence of preproinsulin gene encoding insulin-I and -II was obtained, and the amino acid sequence of A- and B-chains deduced from the nucleotide sequence was identical with that of insulin-I and also insulin-II established by Edman degradation. Furthermore, genomic Southern blot analysis using a part of nucleotide sequence of the preproinsulin gene as a probe showed a single positive band in all cases of genomic DNA digested with each of three restriction endonucleases. These results indicate that insulin-I and -II of barfin flounder arise from a single preproinsulin by proteolytic cleavage at different sites of the signal peptide region.
書誌情報 Zoological science

巻 15, 号 6, p. 931-937, 発行日 1998
ISSN
収録物識別子タイプ ISSN
収録物識別子 02890003
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10545874
DOI
識別子タイプ DOI
関連識別子 info:doi/10.2108/zsj.15.931
フォーマット
内容記述タイプ Other
内容記述 application/pdf
日本十進分類法
主題Scheme NDC
主題 480
出版者
出版者 Zoological Society of Japan
出版者別名
日本動物学会
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