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Preparation of two recombinant crustacean hyperglycemic hormones from the giant freshwater prawn Macrobrachium rosenbergii, and their hyperglycemic activities
http://hdl.handle.net/2261/8078
http://hdl.handle.net/2261/8078274fb83d-0784-47d5-8f67-d94452c7abc5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ZS23_383.pdf (479.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-12-14 | |||||
| タイトル | ||||||
| タイトル | Preparation of two recombinant crustacean hyperglycemic hormones from the giant freshwater prawn Macrobrachium rosenbergii, and their hyperglycemic activities | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Crustacea | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | eyestalk hormone | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | hyperglycemic activity | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | recombinant peptide | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | sinus gland | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | X-organ | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Ohira, Tsuyoshi
× Ohira, Tsuyoshi× Tsutsui, Naoaki× Nagasawa, Hiromichi× Wilder, Marcy N. |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 105917 | |||||
| 姓名 | 長澤, 寛道 | |||||
| 著者所属 | ||||||
| 著者所属 | Japan International Research Center for Agricultural Sciences | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Crustacean hyperglycemic hormone (CHH) is released from the X-organ/sinus gland complex located in the eyestalks, and regulates glucose levels in the hemolymph. In the giant freshwater prawn (Macrobrachium rosenbergii), two cDNAs encoding different CHH molecules were previously cloned by other workers. One of these (Mar-CHH-2) was expressed only in the eyestalks, whereas the other (Mar-CHH-L) was expressed in the heart, gills, antennal gland, and thoracic ganglion, but not in the eyestalks. However, their biological activities had not yet been characterized. Therefore, in this study, recombinant Mar-CHH-2 (rMar-CHH-2) and Mar-CHH-L (rMar-CHH-L) were produced using an E. coli expression system, by expression in bacterial cells and recovery in the insoluble fraction. Thereafter, rMar-CHH-2 and rMar-CHH-L were subjected to refolding and were subsequently purified by reversed-phase HPLC. The rMar-CHH-2 and rMar-CHH-L thus obtained exhibited the same disulfide bond arrangements as those of other CHHs reported previously, indicative of natural conformation. In in vivo bioassay, rMar-CHH-2 showed significant hyperglycemic activity, whereas rMar-CHH-L had no effect. These results indicate that Mar-CHH-L does not function as a CHH, but may have some other, unknown function. | |||||
| 書誌情報 |
Zoological science 巻 23, 号 4, p. 383-391, 発行日 2006 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 02890003 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10545874 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.2108/zsj.23.383 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 480 | |||||
| 出版者 | ||||||
| 出版者 | Zoological Society of Japan | |||||
| 出版者別名 | ||||||
| 日本動物学会 | ||||||
