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Genetic diversity of Pseudo-nitzschia pungens (Bacillariophyceae) in Borneo, Malaysia
https://doi.org/10.15083/00040633
https://doi.org/10.15083/000406333552aabc-1ca8-466b-a704-ddac645bf4bc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
CMS350108.pdf (863.8 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-05-25 | |||||
| タイトル | ||||||
| タイトル | Genetic diversity of Pseudo-nitzschia pungens (Bacillariophyceae) in Borneo, Malaysia | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Pseudo-nitzschia pungens | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ITS | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | rDNA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | population genetic | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | haplotype | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.15083/00040633 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
Lim, Hong Chang
× Lim, Hong Chang× Lim, Po-Teen× Su, Suriyanti Nyun Pau× Teng, Sing Tung× Leaw, Chui-Pin |
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| 著者所属 | ||||||
| 著者所属 | Faculty of Resource Science and Technology, Universiti Malaysia Sarawak | |||||
| 著者所属 | ||||||
| 著者所属 | Institute of Biodiversity and Environmental Conservation, Universiti Malaysia Sarawak | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Pseudo-nitzschia pungens is a chain forming cosmopolitan marine pennate diatom. It is known to produce the neurotoxin, domoic acid (DA) which is responsible for amnesic shellfish poisoning (ASP). In this study, clonal cultures of P. pungens were established from Sabah and Sarawak. To examine the genetic diversity of P. pungens, the ITS region of rDNA was amplified. Nucleotide sequences obtained were used in secondary structure modeling based on homologous modeling and free energy minimization. The ITS sequences of P. pungens showed common structure of ITS1 and ITS2 transcripts. Universal motifs were found in the ITS2 transcript with pyrimidine-pyrimidine (U-U) mismatch and AAA motif between Helix II-III and UGGU motif in the Helix III. Unambiguous orthologous structural sequence alignment was subsequently used in genetic population analyses. Seven haplotypes were found in the Borneon populations. Kuching haplotype is exclusive from the six haplotypes found in Kudat population. The Kuching and Kudat strains formed two distinctive groups inferred by the Profile Neigboring Joining (PNJ) tree. High FST value (>0.69) suggested no gene flow among the populations of P. pungens in Sabah and Sarawak. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Proceedings of the Horiba International Conference "New Direction of Ocean Research in the Western Pacific" : Past, Present and Future of UNESCO/IOC/WESTPAC Activity for 50 years and the JSPS Project "Coastal Marine Science" | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Section I: Research Articles/Harmful microalgae | |||||
| 書誌情報 |
Coastal marine science 巻 35, 号 1, p. 58-63, 発行日 2012-03-20 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13493000 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11957899 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 452 | |||||
| 出版者 | ||||||
| 出版者 | International Coastal Research Center, Atmosphere and Ocean Research Institute, the University of Tokyo | |||||
| 出版者別名 | ||||||
| 東京大学大気海洋研究所国際沿岸海洋研究センター | ||||||
