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  1. 139 大気海洋研究所
  2. 80 国際沿岸海洋研究センター
  3. Coastal Marine Science
  4. 35
  5. 1
  1. 0 資料タイプ別
  2. 30 紀要・部局刊行物
  3. Coastal Marine Science
  4. 35
  5. 1

Genetic diversity of Pseudo-nitzschia pungens (Bacillariophyceae) in Borneo, Malaysia

https://doi.org/10.15083/00040633
https://doi.org/10.15083/00040633
3552aabc-1ca8-466b-a704-ddac645bf4bc
名前 / ファイル ライセンス アクション
CMS350108.pdf CMS350108.pdf (863.8 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2012-05-25
タイトル
タイトル Genetic diversity of Pseudo-nitzschia pungens (Bacillariophyceae) in Borneo, Malaysia
言語
言語 eng
キーワード
主題Scheme Other
主題 Pseudo-nitzschia pungens
キーワード
主題Scheme Other
主題 ITS
キーワード
主題Scheme Other
主題 rDNA
キーワード
主題Scheme Other
主題 population genetic
キーワード
主題Scheme Other
主題 haplotype
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.15083/00040633
ID登録タイプ JaLC
著者 Lim, Hong Chang

× Lim, Hong Chang

WEKO 93380

Lim, Hong Chang

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Lim, Po-Teen

× Lim, Po-Teen

WEKO 93381

Lim, Po-Teen

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Su, Suriyanti Nyun Pau

× Su, Suriyanti Nyun Pau

WEKO 93382

Su, Suriyanti Nyun Pau

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Teng, Sing Tung

× Teng, Sing Tung

WEKO 93383

Teng, Sing Tung

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Leaw, Chui-Pin

× Leaw, Chui-Pin

WEKO 93384

Leaw, Chui-Pin

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著者所属
著者所属 Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
著者所属
著者所属 Institute of Biodiversity and Environmental Conservation, Universiti Malaysia Sarawak
抄録
内容記述タイプ Abstract
内容記述 Pseudo-nitzschia pungens is a chain forming cosmopolitan marine pennate diatom. It is known to produce the neurotoxin, domoic acid (DA) which is responsible for amnesic shellfish poisoning (ASP). In this study, clonal cultures of P. pungens were established from Sabah and Sarawak. To examine the genetic diversity of P. pungens, the ITS region of rDNA was amplified. Nucleotide sequences obtained were used in secondary structure modeling based on homologous modeling and free energy minimization. The ITS sequences of P. pungens showed common structure of ITS1 and ITS2 transcripts. Universal motifs were found in the ITS2 transcript with pyrimidine-pyrimidine (U-U) mismatch and AAA motif between Helix II-III and UGGU motif in the Helix III. Unambiguous orthologous structural sequence alignment was subsequently used in genetic population analyses. Seven haplotypes were found in the Borneon populations. Kuching haplotype is exclusive from the six haplotypes found in Kudat population. The Kuching and Kudat strains formed two distinctive groups inferred by the Profile Neigboring Joining (PNJ) tree. High FST value (>0.69) suggested no gene flow among the populations of P. pungens in Sabah and Sarawak.
内容記述
内容記述タイプ Other
内容記述 Proceedings of the Horiba International Conference "New Direction of Ocean Research in the Western Pacific" : Past, Present and Future of UNESCO/IOC/WESTPAC Activity for 50 years and the JSPS Project "Coastal Marine Science"
内容記述
内容記述タイプ Other
内容記述 Section I: Research Articles/Harmful microalgae
書誌情報 Coastal marine science

巻 35, 号 1, p. 58-63, 発行日 2012-03-20
ISSN
収録物識別子タイプ ISSN
収録物識別子 13493000
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11957899
日本十進分類法
主題Scheme NDC
主題 452
出版者
出版者 International Coastal Research Center, Atmosphere and Ocean Research Institute, the University of Tokyo
出版者別名
東京大学大気海洋研究所国際沿岸海洋研究センター
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