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Genes differentially expressed in testis of a marine fish, the mummichog (Fundulus heteroclitus) after exposure to tributyltin oxide
https://doi.org/10.15083/00040715
https://doi.org/10.15083/0004071511a45e11-5268-42fc-9285-bb37203e9129
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
CMS320109.pdf (1.1 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-02-18 | |||||
| タイトル | ||||||
| タイトル | Genes differentially expressed in testis of a marine fish, the mummichog (Fundulus heteroclitus) after exposure to tributyltin oxide | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | apoptosis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | bromodeoxyuridine | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cDNA subtraction | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | differential display | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | real-time PCR | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | teleost | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.15083/00040715 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
MOCHIDA, Kazuhiko
× MOCHIDA, Kazuhiko× ITO, Katsutoshi× ONDUKA, Toshimitsu× KAKUNO, Akira× FUJII, Kazunori |
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| 著者所属 | ||||||
| 著者所属 | Fisheries Research Agency, National Research Institute of Fisheries and Environment of Inland Sea | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | There is still concern about the effects of organotin compounds (OTs) on marine organisms, and especially on their reproductive systems. We investigated the toxicity of tributyltin oxide (TBTO) on spermatogenesis in a marine fish, the mummichog, Fundulus heteroclitus. The primary purpose of this study is to find biomarkers for evaluation of the effects of TBTO on fish spermatogenesis. We used cDNA subtraction and differential display methods to clone genes differentially expressed in the mummichog testis by TBTO exposure. Six downregulated genes have been identified to date. A GenBank homology search revealed that these genes were homologous to the arginase II gene, a testicular type of creatine kinase gene, two different types of dynein heavy-chain genes, a mitochondrial carrier gene, and a functionally unidentified gene. Real-time PCR revealed that exposure of the fish to 4m g/L of TBTO significantly reduced expression of at least three of the six genes: those encoding creatine kinase, a dynein heavy chain, and the functionally unidentified gene in the testis. In the 4m g/L exposure group, significant reduction in spermatogenic cell proliferation and significant increase in numbers of apoptotic germ cells in the testis were observed. These genes can therefore be used as biomarkers for evaluating the effects of TBTO on fish spermatogenesis. | |||||
| 書誌情報 |
Coastal marine science 巻 32, 号 1, p. 57-66, 発行日 2008-08-20 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13493000 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11957899 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 452 | |||||
| 出版者 | ||||||
| 出版者 | International Coastal Research Center, Ocean Research Institute, the University of Tokyo | |||||
| 出版者別名 | ||||||
| 東京大学海洋研究所国際沿岸海洋研究センター | ||||||
