{"_buckets": {"deposit": "0bb4f8a4-8265-4a2b-9687-4be50b906030"}, "_deposit": {"id": "5594", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "5594"}, "status": "published"}, "_oai": {"id": "oai:repository.dl.itc.u-tokyo.ac.jp:00005594"}, "item_7_biblio_info_7": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-06-27", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_7_date_granted_25": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5", "attribute_value_mlt": [{"subitem_dategranted": "2011-06-27"}]}, "item_7_degree_grantor_23": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u6a5f\u95a2", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "University of Tokyo (\u6771\u4eac\u5927\u5b66)"}]}]}, "item_7_degree_name_20": {"attribute_name": "\u5b66\u4f4d\u540d", "attribute_value_mlt": [{"subitem_degreename": "\u535a\u58eb(\u751f\u547d\u79d1\u5b66)"}]}, "item_7_description_5": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Mrc1/Claspin are evolutionally conserved and has been known to play a major role in replication checkpoint regulation. Furthermore, checkpoint-independent roles at a replication fork have been suggested in yeasts. However, the information on biochemical functions of Claspin has been limited, due to difficulties associated with purification of this protein. I have improved expression and purification procedures for human Claspin as well as other high molecular weight replication proteins. I used a CSII-EF-MCS expression vector and Polyethyleneimine (PEI) as a transfection reagent for high-level expression in 293T cells. This method could be generally applicable to purification of large-sized proteins which have been difficult to handle. Purified Claspin stimulated the helicase activity of the MCM4-6-7 complex in vitro. This finding can at least partially explain a stimulatory role of Claspin at a replication fork. I also identified interactions of Claspin with replication and checkpoint factors, which may bear physiological significance.", "subitem_description_type": "Abstract"}]}, "item_7_dissertation_number_26": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7", "attribute_value_mlt": [{"subitem_dissertationnumber": "\u7532\u7b2c27368\u53f7"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "11646", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u5b87\u91ce, \u4fee\u53f8"}]}]}, "item_7_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.15083/00005585", "subitem_identifier_reg_type": "JaLC"}]}, "item_7_select_21": {"attribute_name": "\u5b66\u4f4d", "attribute_value_mlt": [{"subitem_select_item": "doctoral"}]}, "item_7_subject_13": {"attribute_name": "\u65e5\u672c\u5341\u9032\u5206\u985e\u6cd5", "attribute_value_mlt": 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"\u6771\u4eac\u5927\u5b66\u5927\u5b66\u9662\u65b0\u9818\u57df\u5275\u6210\u79d1\u5b66\u7814\u7a76\u79d1\u751f\u547d\u79d1\u5b66\u7814\u7a76\u7cfb\u30e1\u30c7\u30a3\u30ab\u30eb\u30b2\u30ce\u30e0\u5c02\u653b"}, {"subitem_text_value": "Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Uno, Syuzi"}], "nameIdentifiers": [{"nameIdentifier": "11645", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-06-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "K-03492.pdf", "filesize": [{"value": "2.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": 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