{"_buckets": {"deposit": "eeb54050-24e8-4adc-b535-5bda808b0b18"}, "_deposit": {"id": "661", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "661"}, "status": "published"}, "_oai": {"id": "oai:repository.dl.itc.u-tokyo.ac.jp:00000661"}, "item_2_biblio_info_7": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-06-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageStart": "e98737", "bibliographicVolumeNumber": "9", "bibliographic_titles": [{"bibliographic_title": "PLoS ONE"}]}]}, "item_2_description_5": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "WRKY transcription factors and mitogen-activated protein kinase (MAPK) cascades have been shown to play pivotal roles in the regulation of plant defense responses. We previously reported that OsWRKY53-overexpressing rice plants showed enhanced resistance to the rice blast fungus. In this study, we identified OsWRKY53 as a substrate of OsMPK3/OsMPK6, components of a fungal PAMP-responsive MAPK cascade in rice, and analyzed the effect of OsWRKY53 phosphorylation on the regulation of basal defense responses to a virulence race of rice blast fungus Magnaporthe oryzae strain Ina86-137. An in vitro phosphorylation assay revealed that the OsMPK3/OsMPK6 activated by OsMKK4 phosphorylated OsWRKY53 recombinant protein at its multiple clustered serine-proline residues (SP cluster). When OsWRKY53 was coexpressed with a constitutively active mutant of OsMKK4 in a transient reporter gene assay, the enhanced transactivation activity of OsWRKY53 was found to be dependent on phosphorylation of the SP cluster. Transgenic rice plants overexpressing a phospho-mimic mutant of OsWRKY53 (OsWRKY53SD) showed further-enhanced disease resistance to the blast fungus compared to native OsWRKY53-overexpressing rice plants, and a substantial number of defense-related genes, including pathogenesis-related protein genes, were more upregulated in the OsWRKY53SD-overexpressing plants compared to the OsWRKY53-overexpressing plants. These results strongly suggest that the OsMKK4-OsMPK3/OsMPK6 cascade regulates transactivation activity of OsWRKY53, and overexpression of the phospho-mimic mutant of OsWRKY53 results in a major change to the rice transcriptome at steady state that leads to activation of a defense response against the blast fungus in rice plants.", "subitem_description_type": "Abstract"}]}, "item_2_description_6": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "UTokyo Research\u63b2\u8f09\u300c\u30a4\u30cd\u306e\u8010\u75c5\u6027\u5411\u4e0a\u306b\u6210\u529f\u300d URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/", "subitem_description_type": "Other"}, {"subitem_description": "UTokyo Research \"Enhanced disease resistance in rice plants\" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/", "subitem_description_type": "Other"}]}, "item_2_publisher_20": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "PLOS"}]}, "item_2_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1371/journal.pone.0098737", "subitem_relation_type_select": "DOI"}}]}, "item_2_relation_25": {"attribute_name": "\u95a2\u4fc2URI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/", "subitem_relation_type_select": "URI"}}, {"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/", "subitem_relation_type_select": "URI"}}, {"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0098737", "subitem_relation_type_select": "URI"}}]}, "item_2_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "(c) 2014 Chujo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]}, "item_2_source_id_8": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1932-6203", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_21": {"attribute_name": "\u51fa\u7248\u8005\u5225\u540d", "attribute_value_mlt": [{"subitem_text_value": "Public Library of Science"}]}, "item_2_text_34": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_text_value": "Journal Article"}]}, "item_2_text_4": {"attribute_name": "\u8457\u8005\u6240\u5c5e", "attribute_value_mlt": [{"subitem_text_value": "Biotechnology Research Center, The University of Tokyo"}, {"subitem_text_value": "Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences"}, {"subitem_text_value": "Department of Biosciences, Teikyo University, Utsunomiya"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Chujo, Tetsuya"}], "nameIdentifiers": [{"nameIdentifier": "2192", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miyamoto, Koji"}], "nameIdentifiers": [{"nameIdentifier": "2193", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogawa, Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "2194", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Masuda, Yuka"}], "nameIdentifiers": [{"nameIdentifier": "2195", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimizu, Takafumi"}], "nameIdentifiers": [{"nameIdentifier": "2196", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kishi-Kaboshi, Mitsuko"}], "nameIdentifiers": [{"nameIdentifier": "2197", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takahashi, Akira"}], "nameIdentifiers": [{"nameIdentifier": "2198", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishizawa, Yoko"}], "nameIdentifiers": [{"nameIdentifier": "2199", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Minami, Eiichi"}], "nameIdentifiers": [{"nameIdentifier": "2200", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nojiri, Hideaki"}], "nameIdentifiers": [{"nameIdentifier": "2201", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamane, Hisakazu"}], "nameIdentifiers": [{"nameIdentifier": "2202", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okada, Kazunori"}], "nameIdentifiers": [{"nameIdentifier": "2203", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-05-30"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "journal.pone.0098737.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "journal.pone.0098737.pdf", "url": "https://repository.dl.itc.u-tokyo.ac.jp/record/661/files/journal.pone.0098737.pdf"}, "version_id": "84552a52-28e4-4116-8cc0-697c1c239314"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice"}]}, "item_type_id": "2", "owner": "1", "path": ["144/145", "9/10/11"], "permalink_uri": "http://hdl.handle.net/2261/55990", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2015-02-04"}, "publish_date": "2015-02-04", "publish_status": "0", "recid": "661", "relation": {}, "relation_version_is_last": true, "title": ["Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice"], "weko_shared_id": null}